文献类型：期刊文献

中文题名：杨树维管组织特异启动子的克隆与启动活性分析

英文题名：Cloning and Functional Analysis of a Vascular Tissue-specific Promoter from Populus tomentosa

作者：贺郭[1] 王敏杰[1] 陈洪亮[1,2] 赵树堂[1] 卢孟柱[1]

第一作者：贺郭

机构：[1]中国林业科学研究院林业研究所,国家林业局林木培育重点实验室,北京100091;[2]北京大学生命科学学院,北京100871

年份：2010

期号：2

起止页码：177-183

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家863计划项目(2006AA100109);国际先进农业科学技术(948)重大创新项目(2006-4-C01)

语种：中文

中文关键词：维管组织;启动子;组织特异;GUS染色;基因工程

外文关键词：vascular tissue ; promoter; tissue-specific ; GUS stain ; genetic engineering

分类号：S792.11

摘要：启动子在基因表达调控中起关键性作用,它在很大程度上决定所控基因表达的时间、空间和强度。依据拟南芥ATH1芯片分析杨树维管形成时期特异表达基因的结果,选取了差异表达基因NST3,通过BLAST比对在杨树EST数据库(PopulusDB)中找到同源性较高的基因NAC068。以毛白杨为材料,在其基因组中克隆得到该基因5'侧翼区901 bp长的片段,命名为pProNAC068,将该片段置换pBI121载体中的CaMV35S启动子,并在84K杨中检测报告基因GUS的表达情况。经过GUS活性检测分析发现:该启动子可以控制外源基因在次生维管组织中特异表达,从而为基因工程中有目的的控制外源基因在维管组织中的表达奠定基础。
Promoter plays a .key role not only in regulating the level of gene expression but also in controlling the expression in a spatial and temporal manner. According to the expression profile generated using Arabidopsis ATH1 genechip to analyze genes involved in the regeneration of the secondary vascular system in Populus tomentosa Carr, a differentially expressed gene NST3 was chosen and its homologous gene NAC068 in poplar were found using BLAST software against poplar EST database （PopulusDB）. Then the 5＇- upstream sequence of this gene was cloned from Populus tomentosa Cart. and put into the plant expression vector pBI121 to replace the 35S promoter to control the reporter gene gus. After transformation of polar 84K via Agrobactera, the GUS activity was detected in cambium zone, suggesting this promoter could control gene expression in secondary vascular system thus should be useful in genetic engineering to control exogenous gene specific expression in vascular system.