文献类型：期刊文献

英文题名：Amphioxus IGF-like peptide induces mouse muscle cell development via binding to IGF receptors and activating MAPK and PI3K/Akt signaling pathways

作者：Liu, Mingying[2,3] Zhang, Shicui[1,2]

第一作者：刘明英;Liu, Mingying

通信作者：Zhang, SC[1]

机构：[1]Ocean Univ China, Dept Marine Biol, Qingdao 266003, Peoples R China;[2]Ocean Univ China, Inst Evolut & Marine Biodivers, Qingdao 266003, Peoples R China;[3]Chinese Acad Forestry, Inst Subtrop Forestry, State Key Lab Forest Genet & Tree Breeding, Beijing, Peoples R China

年份：2011

卷号：343

期号：1-2

起止页码：45-54

外文期刊名：MOLECULAR AND CELLULAR ENDOCRINOLOGY

收录：;Scopus(收录号：2-s2.0-80051575880);WOS:【SCI-EXPANDED(收录号:WOS:000295349200006)】；

基金：This work was supported by grants of Natural Science Foundation of China (NSCF; 30972274) and Ministry of Science and Technology (MOST; 2008AA092603). The authors are also grateful to Dr. WG Yu in School of Medicine and Pharmacy, OUC, for technical help in Western blotting.

语种：英文

外文关键词：Amphioxus; Lancelet; Branchiostoma; IGF; IGF receptor; Muscle cells

摘要：Insulin-like growth factors (IGFs) are identified in all vertebrates. An insulin/IGF hybrid polypeptide has also been identified in protochordate amphioxus. However, whether this hybrid polypeptide functions as vertebrate IGFs remains unknown. Here we established a primary culture system of mouse muscle satellite cells as an in vitro model to investigate the effects of amphioxus IGF-like molecule on muscle cell development. Like human IGF, recombinant IGF-like molecule was able to stimulate the proliferation of mouse muscle cells. Besides, it was able to bind to the cells and the partially purified IGF receptors from mouse muscle cells. Moreover, recombinant IGF-like molecule was capable of activating MAPK and PI3K/Akt pathways by stimulating phosphorylation of MAPK and Akt. This interaction of amphioxus IGF-like molecule with mammalian (mouse) IGF receptors and its induction of similar downstream signaling pathways add substantially to the hypothesis of the presence of IGF signaling in the common ancestor of protochordate/vertebrates. (C) 2011 Elsevier Ireland Ltd. All rights reserved.