文献类型：期刊文献

英文题名：An evolutionary conserved CBL1-CIPK6 complex of oil persimmon involved in responses to ABA, salt and drought stress

作者：Liu, Cuiyu[1,2] Yang, Xu[1,2] Jiang, Xibing[1,2] Song, Juan[3] Xu, Yang[1,2]

第一作者：刘翠玉;Liu, Cuiyu

通信作者：Xu, Y[1];Xu, Y[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[2]Zhejiang Prov Key Lab Tree Breeding, Hangzhou 311400, Peoples R China;[3]Huzhou Normal Univ, Huzhou 313000, Peoples R China

年份：2025

卷号：26

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001665478300005)】；

基金：This research was supported by the Zhejiang Provincial Natural Science Foundation of China (LQ24C160001); Key Agricultural New Varieties Breeding Projects of the Zhejiang Province Science and Technology Department (2021C02066-10); National Key R&D Program of China (2024YFD2200600).

语种：英文

外文关键词：CBL-CIPK complex; Evolutionary conserved; Stress responses; ROS-scavenging; Ion transport

摘要：BackgroundsCalcineurin B-like proteins (CBLs) interact with CBL-interacting protein kinases (CIPKs) to form CBL-CIPK complexes, which regulate plant responses to biotic/abiotic stresses. These complexes originated from a common ancestor and subsequently diverged during evolution of land plants. In our previous work, a pair of CBL-CIPK genes from oil persimmon (Diospyros oleifera Cheng) were identified to play different roles in plant stress responses. However, their evolutionary trajectories, biological functions and regulatory networks remain unclear.ResultsIn this work, the cloned CBL and CIPK genes from oil persimmon were named as DoCBL1 and DoCIPK6 based on their homology. The phylogenetic analysis and sequence alignment showed that CBL1 and CIPK6 proteins are evolutionarily and structurally conserved across various plant species. The expansion of these proteins in angiosperms might enhance plant adaptation to abiotic stress. Additionally, DoCBL1 and DoCIPK6 proteins were localized at the plasma membrane in tobacco (Nicotiana tabacum L.) leaves, and DoCBL1 interacted with DoCIPK6 in both yeast two-hybrid (Y2H) and bimolecular fluorescence complementation (BiFC) assays. Furthermore, the potential functions of DoCBL1 and DoCIPK6 were explored by mutating and overexpressing them in Arabidopsis thaliana (Columbia), using wild-type plants as controls, under ABA, salt and drought stress. The results revealed that DoCBL1 and DoCIPK6 transgenic plants exhibited enhanced tolerance to salt and drought stress. This tolerance was achieved by altering the expression of ABI1, AKT1, CAX1, SOS1/2/3, PP2CA, DREB1A and RD26, thereby regulating the balance of K+, Ca2+ and Na+, as well as ROS-scavenging activities. Meanwhile, the transgenic Arabidopsis exhibited increased sensitivity to ABA, characterizing by elevated contents of Na+ and reactive oxygen species (ROS), including H2O2, O-2(-), and MDA. These plants also showed significant reductions in biomass, K+ content, and the activities of SOD, POD and CAT.ConclusionsOur findings suggested that DoCBL1 and DoCIPK6 might confer enhanced salt and drought tolerance, as well as increased sensitivity to ABA, in Arabidopsis plants. We proposed that DoCBL1-DoCIPK6 complex serves as a conserved bridge between stress signals and downstream transcriptional regulation, and thereby offering a novel perspective on their roles in abiotic stress responses.