文献类型：期刊文献

中文题名：湿加松外植体消毒及初始培养基筛选研究

英文题名：Study on Explants Sterilization and Initial Culture Medium Selection of Pinus elliottii×P.caribaea

作者：常金财[1,2] 宗亦臣[1] 郑勇奇[1] 白淑兰[2]

第一作者：常金财

机构：[1]中国林业科学研究院林业研究所;[2]内蒙古农业大学林学院

年份：2010

卷号：26

期号：3

起止页码：129-133

中文期刊名：中国农学通报

外文期刊名：Chinese Agricultural Science Bulletin

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：科技部林业科技支撑计划"高产优质多抗松杉新品种选育"专题(2006BAD01A1405);中央级公益性科研院所专项资金项目"杂交松无性系快繁技术研究"(200713);国家科技基础条件平台"外来树种种质资源标准化整理整合与共享"(2005DKA21003-8)的部分研究内容

语种：中文

中文关键词：湿加松;消毒;初始培养基;组培

外文关键词：Pinus elliottii×P.caribaea; sterilization; initial culture medium; tissue culture

分类号：S792.19

摘要：以2年生澳大利亚种源湿加松茎尖为试材,研究了不同消毒程序防控外植体污染的效果,得出最佳消毒程序为茎尖清水冲洗5 min,用含表面活化剂吐温-20的无菌水冲洗1 min,70%乙醇消毒10 s,0.1%HgCl_2浸泡6.5 min,无菌水冲洗5遍。以MS、1/2MS、GD、DCR、WPM、N_6等6种针叶树常用培养基,细胞分裂素6-BA、生长素NAA进行湿加松基本培养基的筛选,调查芽的诱导、增殖、伸长和生根的情况。筛选出湿加松初始培养基为GD+BA(0.1～1.0 mg/L)+NAA(0.1～0.4 mg/L)。
This study intended to establish a culture system that would induce explant sterilization and initial medium.The stem of hybrid pine was 2 years old,which seeds were made of Pinus elliottii across Pinus caribaea of Australia.The results showed that the best way of explants sterilization was as follows：stem was washed in water for 5 minutes,in sterile water with tween-20 for 1 minute,in 70%ethanol for 10 seconds,in 0.1%HgCl2 for 6.5 minutes,and washed in sterile water for 5 times.Selection of the initial culture medium was with MS,1/2 MS,GD,DCR,WPM and N6,which were common culture mediums on pine.6-BA and NAA were also added to the selected medium.Inductivity,proliferation,elongation and root rate of bud were as the evaluating indicator. The better culture medium of hybrid pine was GD＋BA（0.1-1.0mg/L）＋NAA（0.1-0.4 mg/L）.