文献类型：期刊文献

中文题名：奇楠沉香精油化学成分及其生物活性分析

英文题名：Chemical Components and Bioactivity of Qi-nan Agarwood Essential Oils

作者：晏婷婷[1] 马生[1] 陈媛[1] 李改云[1]

第一作者：晏婷婷

机构：[1]中国林业科学研究院木材工业研究所,北京100091

年份：2024

卷号：44

期号：6

起止页码：57-63

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;Scopus;北大核心:【北大核心2023】；CSCD:【CSCD2023_2024】；

基金：中国林业科学研究院中央基本业务科研经费青年协同创新项目(CAFYBB2021QD003);海南省重点研发项目(SQ2022SHFZ0099)。

语种：中文

中文关键词：新种质白木香;奇楠沉香精油;化学成分;生物活性

外文关键词：new germplasm of Aquilaria sinensis;Qi-nan agarwood essential oil;chemical components;bioactivity

分类号：TQ35

摘要：以实验室水蒸馏提取的普通沉香和奇楠沉香精油,以及市场中购买的不同提取方法的奇楠沉香精油为研究对象,使用GC-MS分析其主要化学成分,采用HPLC对2个主要色酮成分进行含量测定,并通过二维薄层色谱-定向功能分析(2D-TLC-EDA)结合二级质谱确认精油中具有抗氧化活性和酪氨酸酶抑制活性的主要成分。研究结果显示:不同方法提取的奇楠沉香精油中主要成分除倍半萜和芳香族类化合物外,还含有2-(2-苯乙基)色酮类,2个最主要的2-(2苯乙基)色酮化合物的含量与提取方法相关。在水蒸馏提取的精油中含量最低,质量分数为0.57%~2.88%;超临界分级提取的精油中含量则较高,质量分数为14.13%~86.59%,色酮含量增加使精油密度增加。奇楠沉香精油中的2-(2-(3-羟基-4-甲氧基苯基)乙基)色酮能够清除DPPH自由基(DPPH·),2-(2-(4-甲氧基苯基)乙基)色酮和2-(2苯乙基)色酮具酪氨酸酶抑制活性。
In this paper,the essential oils of common agarwood and Qi-nan agarwood extracted by hydro-distillation in the lab and the essential oils of Qi-nan agarwood purchased by different extraction methods in the market were taken as the research objects to analyze the chemical components and bioactivity of agarwood essential oils from a new germplasm of Aquilaria sinensis.The main chemical components were analyzed by GC-MS and the contents of main 2-(2-phenylethyl)chromones(PECs)were determined by HPLC.The main components with antioxidant activity and tyrosinase inhibitory activity in essential oil were identified with two dimensional-thin layer chromatography-effect directed functional analysis(2D-TLC-EDA)combined with liquid chromatography-mass spectrometry(LC-MS/MS).The results showed that besides sesquiterpenoids and aromatic compounds,the agarwood essential oils from different extraction methods also contained 2-(2-phenethyl)chromones.The contents of the main 2-(2-phenylethyl)chromone compounds were related to the extraction methods,and the content of the essential oil extracted by hydro-distillation were the lowest with a mass fraction of 0.57%-2.88%,while they were higher with a mass fraction of 14.13%-86.59%in essential oil extracted by supercritical fluid extraction.The increase in the contents of chromones led to an increase in the density of the essential oil.2-(2-(3-hydroxy-4-methoxyphenyl)ethyl)chromone had DPPH free radical(DPPH·)scavenging ability,and 2-(2-phenylethyl)chromone and 2-(2-(4-methoxyphenyl)ethyl)chromone possessed tyrosinase inhibitory activity.