文献类型：期刊文献

英文题名：Abnormal meiotic chromosome behavior and gametic variation induced by intersectional hybridization in Populus L.

作者：Wang, Jun[1,2,3] You, Hanli[1,2] Tian, Ju[4] Wang, Yongfu[5] Liu, Minghu[6] Duan, Wula[6]

第一作者：Wang, Jun

通信作者：Wang, J[1]

机构：[1]Beijing Forestry Univ, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China;[2]Beijing Forestry Univ, MOE, Key Lab Genet & Breeding Forest Trees & Ornamenta, Beijing 100083, Peoples R China;[3]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China;[4]Beijing Forestry Univ, Acad Forestry, Beijing 100083, Peoples R China;[5]Forestry Res Inst Tongliao City, Tongliao 028000, Inner Mongolia, Peoples R China;[6]Chinese Acad Forestry, Expt Ctr Desert Forestry, Dengkou Cty 015200, Inner Mongolia, Peoples R China

年份：2015

卷号：11

期号：3

起止页码：1-11

外文期刊名：TREE GENETICS & GENOMES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000355704700029)】；

基金：This work was supported by the Beijing Higher Education Young Elite Teacher Project (Grant No. YETP0758), the Program for New Century Excellent Talents in University (Grant No. NCET-13-0672), the Program for Changjiang Scholars and Innovative Research Team in University (Grant No. IRT13047), the Beijing Nova program (Grant No. Z141105001814007), and the Students' Innovation and Entrepreneurship Training Program of BJFU (Grant No. X201410022032).

语种：英文

外文关键词：Distant hybrid; Meiosis; Microtubular cytoskeleton; Populus simoniix P. euphratica Pollen sterility; Unreduced pollen

摘要：Abnormal meiotic chromosome behavior and pollen variation in two intersectional hybrids between Populus simonii Carr. (section Tacamahaca Spach) and Populus euphratica Oliv. (section Turanga Bge.) were investigated by the squashed technique and indirect immunofluorescence. Both hybrids produced abnormal flower buds characterized by a primary bud with an accessary bud at frequencies of 39.0 and 17.8 %, respectively. Chromosome pairing analysis at late diakinesis showed average configurations 20.31 I+7.04 II+1.42 III+0.04 IV in hybrid "Xhu-DK" and 19.18 I+7.16 II+1.45 III+0.04 IV in hybrid "Xhu-TL", indicating high divergence between their parent species. Meiotic abnormalities, including precocious chromosome migration, lagging chromosomes, chromosome bridges, and micronuclei, were observed at high frequency, resulting in imbalanced chromosome segregation and chromosome elimination and caused high gamete sterility. Some micronuclei triggered formation of minispindles in Metaphase II, which were responsible for the formation of microcytes. Fused and tripolar spindles in the second meiotic division developed into dyads and triads, respectively, leading to viable first meiotic division restitution (FDR) type unreduced pollen formation. However, Xhu-TL was completely sterile because the high frequency of precocious cytokinesis during the second division prevented unreduced pollen formation though spindle fusion. Xhu-DK produced 29.6 % viable pollen grains. The diameter of viable pollen ranged from 19.6 to 64.9 mu m, suggesting that Xhu-DK might be capable of producing unreduced pollen or pollen with a higher ploidy level. The strategy of using the distant hybrids for chromosome introgression and polyploidy breeding in Populus L. was considered.