文献类型：期刊文献

中文题名：人工低温胁迫下多年生黑麦草诱变株系的抗寒性研究

英文题名：Assessing cold resistance of mutagenic strains of perennial ryegrass under artificial low-temperature stress

作者：翟飞飞[1] 韩蕾[1] 刘俊祥[1] 钱永强[1] 巨关升[1] 李伟[1] 张少伟[1] 孙振元[1]

第一作者：翟飞飞

机构：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室

年份：2013

卷号：22

期号：6

起止页码：268-279

中文期刊名：草业学报

外文期刊名：Acta Prataculturae Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家"863"计划项目(2011AA100209)资助

语种：中文

中文关键词：多年生黑麦草;诱变株系;抗寒性;酶活性;渗透调节;叶绿素荧光

外文关键词：perennial ryegrass; mutagenic lines; cold resistance; enzyme activity; osmotic regulation; chlorophyll fluorescence

分类号：S543.601;Q945.79

摘要：本研究以多年生黑麦草‘Derby’的5个诱变株系G3-2、G10-5、G16-1、CK9-3、CK14-3和抗寒性较好的多年生黑麦草‘Pinnacle’为材料,研究其在人工低温驯化过程中生理指标的变化,以对诱变株系的抗寒性作出评价并筛选出抗寒性好的多年生黑麦草。结果显示,在低温驯化过程中,各试材的叶片含水量下降,G16-1的下降幅度最大,为3.12%。细胞膜伤害程度加重,诱变株系中CK14-3的细胞膜透性最小,为25.40%,CK9-3丙二醛含量最低,为38.06μmol/g。渗透调节物质含量增加,CK14-3和‘Pinnacle’的脯氨酸含量最低,分别为0.18%和0.15%;G16-1的可溶性蛋白增加量最大,为83.08%;‘Pinnacle’的可溶性糖增加量最小,为34.18%,CK9-3次之,为53.41%;G10-5和G16-1与‘Pinnacle’的还原性糖含量相当,分别为9.01%和9.79%。抗氧化物酶活性增强,CK14-3的超氧化物歧化酶和过氧化氢酶活性较低,分别为5171.21U/(g·h)和171.04U/(g·min),但变化率较大,分别为169.07%和47.06%,与‘Pinnacle’相当。潜在光化学效率降低,诱变株系中以G3-2和G16-1降幅较小,分别为3.46%和3.44%。经主成分分析与隶属函数分析,各试材的抗寒性从强到弱依次是‘Pinnacle’、CK14-3、G10-5、G16-1、CK9-3、G3-2。采用聚类分析把试材分成3类:‘Pinnacle’的抗寒性强,CK14-3、G10-5、G16-1和CK9-3的抗寒性中等,G3-2的抗寒性差。综合分析可知,在低温驯化过程中,抗寒性越强的多年生黑麦草,其细胞膜相对透性越小,叶片含水量、丙二醛含量、脯氨酸含量和还原性糖含量越低,可溶性蛋白含量、超氧化物歧化酶活性和过氧化氢酶活性的变化率越大,可溶性糖含量和潜在光化学效率的变化率越小。
In order to evaluate the cold resistance of mutagenic lines of perennial ryegrass and obtain more cold resistance perennial ryegrasses, we studied the physiological and biochemical responses of perennial ryegrasses under low temperature （above zero） treatment, and the materials of this experiment are five mutagenic lines- G3-2, G10-5, G16-1, CK9-3, CK14-3 and one variety with good cold resistance-＇Pinnacle＇. The results of this study show that, in the process of cold acclimation, the leaf water content decrease： G16-1＇s leaf water content decreases by up to 3. 12%. Relative membrane permeability increases and malondialdehyde accumulates： CK14-3 has the lowest relative membrane permeability, which is 25.40% ; while CK9-3 accumulates the lowest malondialdehyde content, which is 38. 06 μmol/g. The osmotic regulation substances increase： CK14-3 and ＇Pinnacle＇ have lower proline content with 0.18% and 0.15% respectively; G16-1＇s solute protein content in- creases by 83. 08%, which is the largest; ＇Pinnacle＇ s solute sugar content is the lowest increaseing by 34.18%, and CK9-3 takes the second place with 53.41% ; the reducing sugar content of G10-5 and G16-1 weigh against ＇Pinnacle＇ with 9.01% and 9.79 % separately. The antioxidant enzymes are enhanced, the superoxide dismutase activity and catalase activity of CK14-3 are lower, with5171.21 U/（g · h） and 171.04 U/（g · min） respectively, but the changing rate are quicker with 169. 07% and 47.06% respectively. As to potential photochemical efficiency, G3-2 and G16-1 decreases by 3.46% and 3.44% separately, which are small. By principal component analysis and membership function calculating, the cold-resistance ranking of the experiment materials is ＇Pinnacle＇, G16-1, CK9-3, G10-5, CK14-3, G3-2 from strong to weak. The experiment materials are classified into three types： ＇Pinnacle＇＇s cold-tolerance is strong, G16-1, CK9-3, G10-5 and CK14-3＇s are medium and G3-2＇s is poor. Considering the results, we find that the more cold-resistance the perennial ryegrass is, the less relative membrane permeability it has; the less leaf water content, malondialdehyde content, proline content and reducing sugar content it have, the quicker the soluble protein content, superoxide dismutase and catalase activities change, the slower solute sugar content and potential photochemical efficiency change.