文献类型：期刊文献

中文题名：山沉香结香前后显微组织化学的比较研究

英文题名：Microscopic histochemical comparison during mountain-agarwood formation

作者：高佳琪[1,2] 刘娟[2] 焦立超[3] 殷亚方[3] 柴兴云[4] 陈苏依勒[5] 黄璐琦[1,2]

第一作者：高佳琪

机构：[1]江苏大学药学院,江苏镇江212013;[2]中国中医科学院中药资源中心,北京100700;[3]中国林业科学研究院木材工业研究所,北京100091;[4]北京中医药大学中药现代中心,北京100029;[5]内蒙古阿拉善盟蒙医医院,内蒙古阿拉善750300

年份：2020

卷号：45

期号：16

起止页码：3797-3804

中文期刊名：中国中药杂志

外文期刊名：China Journal of Chinese Materia Medica

收录：CSTPCD;;Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；PubMed;

基金：国家重点研发计划项目(2019YFC1711000);中央本级重大增减支项目(2060302);中央级公益性科研院所基本科研业务费专项资金项目(ZZ13-YQ-093,ZZXT201904)。

语种：中文

中文关键词：山沉香;结香;组织化学;显微观察

外文关键词：Syringa pinnatifolia;mountain-agarwood formation;histochemistry;microscopic observation

分类号：R282.5

摘要：山沉香以其独特的药用价值在我国民族医药应用中发挥着重要作用,现代药理学研究也表明其具有抗心肌缺血、抗菌、抗炎、抗肿瘤、镇痛等功效。山沉香药材取自丁香属植物羽叶丁香的去皮根、茎、粗枝等部位,目前主要根据其横截面的紫色侵填物与香气判断山沉香是否结香,但对其结香机制研究尚未见报道。为了解山沉香结香过程中的微观变化,该文对结香前后的山沉香进行组织化学染色和显微观察,总结山沉香药材的显微结构特征、比较结香前后山沉香的组织化学差异。结果显示,结香前后的山沉香各部位侵填体分布具有显著差异;未结香材料具有较多淀粉粒和活细胞,而结香材料的分布规律相反;山沉香射线细胞的多糖含量和木质化程度随结香程度的加深而增加。该研究表明,符合临床用药要求的山沉香在心材部位含大量紫红色侵填体,黄棕色侵填体在心材与边材都有分布但边材相对较少,射线部位木质化程度很高,具活力细胞少,含大量多糖,几乎无淀粉存在。该研究旨在揭示山沉香结香过程中组织化学特征的改变,为深入探索山沉香的结香机制奠定基础。
Mountain-agarwood plays an important role in ethnic medicine in China for its pharmaceutical value.Modern pharmacological researches demonstrated that mountain-agarwood was effective for its anti-myocardial ischemia,antibacterial,anti-inflammatory,antitumor and analgesic effects.Mountain-agarwood derives from the peeled roots,stems or twigs of Syringa pinnatifolia which belongs to Syringa genus.It often depends on the purple substance and fragrance to estimate the formation of mountain-agarwood.However,the mechanism of mountain-agarwood formation has not been reported.To observe the microcosmic change in the process during the formation of mountain-agarwood,this study described the microscopic and histochemical characteristics of mountain-agarwood formation through histochemical staining.Our results showed that a significant difference of the distribution of tyloses existed during mountain-agarwood formation.It was observed that inchoate mountain-agarwood had more starch granules and viable cells than mountain-agarwood formed with high level or low level.The amount of polysaccharide and degree of lignification were increased during the mountain-agarwood formation.The results indicated that the mountain-agarwood,which meets the quality requirements for pharmaceutical use,contained the following characteristics:a large amount of purple tyloses in heartwood;yellow-brown tyloses distributing in heartwood and sapwood which were less in the latter;lignification with high level;a few viable cells;lots of polysaccharide and few starch granules in xylem rays cell.This study is aimed to reveal the change of histochemical characteristics during mountain-agarwood formation,and lay the foundation for exploring the mechanism of mountain-agarwood formation.