文献类型：期刊文献

英文题名：Overexpression of quinone reductase from Salix matsudana Koidz enhances salt tolerance in transgenic Arabidopsis thaliana

作者：Song, Xixi[1,2] Fang, Jie[1,2] Han, Xiaojiao[1,2] He, Xuelian[1,3] Liu, Mingying[1,2] Hu, Jianjun[1,4] Zhuo, Renying[1,2]

第一作者：Song, Xixi

通信作者：Zhuo, RY[1];Hu, JJ[2]|[a00058eb5603bedf0c2e1]卓仁英;

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Key Lab Tree Breeding Zhejiang Prov, Res Inst Subtrop Forestry, Beijing 100091, Peoples R China;[3]China Three Gorges Univ, Biotechnol Res Ctr, Yichang 443002, Hubei, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, State Forestry Adm, Beijing 100091, Peoples R China

年份：2016

卷号：576

期号：1

起止页码：520-527

外文期刊名：GENE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000370464200018)】；

基金：This work was supported by the Special Fund for Forest Scientific Research in the Public Welfare No. 201304103 and the National Natural Science Foundation of China No. 31370600 and 31300508.

语种：英文

外文关键词：Quinone reductase; Salix matsudana; Salt stress; Overexpression

摘要：Quinone reductase (QR) is an oxidative-related gene and few studies have focused on its roles concerning salt stress tolerance in plants. In this study, we cloned and analyzed the QR gene from Salix matsudana, a willow with tolerance of moderate salinity. The 612-bp cDNA corresponding to SmQR encodes 203 amino acids. Expression of SmQR in Escherichia coli cells enhanced their tolerance under salt stress. In addition, transgenic Arabidopsis thaliana lines overexpressing SmQR exhibited higher salt tolerance as compared with WT, with higher QR activity and antioxidant enzyme activity as well as higher chlorophyll content, lower methane dicarboxylic aldehyde (MDA) content and electric conductivity under salt stress. Nitro blue tetrazolium (NBT) and 3,3'-diaminobenzidine (DAB) staining also indicated that the transgenic plants accumulated less reactive oxygen species compared to WT when exposed to salt stress. Overall, our results suggested that SmQR plays a significant role in salt tolerance and that this gene may be useful for biotechnological development of plants with improved tolerance of salinity. (c) 2015 Elsevier B.V. All rights reserved.