文献类型：期刊文献

英文题名：Transcription factor LkWOX4 is involved in adventitious root development in Larix kaempferi

作者：Wang, Hongming[1,2] Xie, Yunhui[1] Liu, Wusheng[3] Tao, Guiyun[1] Sun, Chao[1] Sun, Xiaomei[1] Zhang, Shougong[1]

第一作者：Wang, Hongming

通信作者：Zhang, SG[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry &, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Tianshui Normal Univ, Coll Bioengn & Biotechnol, Tianshui 741000, Gansu, Peoples R China;[3]North Carolina State Univ, Dept Hort Sci, Raleigh, NC 27695 USA

年份：2020

卷号：758

外文期刊名：GENE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000564694000015)】；

基金：We thank Lesley Benyon, PhD, from Liwen Bianji, Edanz Group China (www.liwenbianji.cn/ac), for editing the English text of a draft of this manuscript. We thank the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2018ZY001-4) and the National Transgenic Major Program (2018ZX08020-003) for funding.

语种：英文

外文关键词：Larch; Adventitious root; LkWOX4; RNA-Seq; Interaction protein

摘要：WUSCHEL-related homeobox4 (WOX4) plays important roles in vascular formation and adventitious root (AR) development. Here, we cloned the WOX4 from the AR of Larix kaempferi, whose cDNA is 1452 bp in length and encodes 483 amino acids. LkWOX4 is mainly expressed in the layer formation area of the stem at 10 days after cutting and its expression levels in the middles and ends of the ARs were higher than that in the AR tips. The fused protein LkWOX4-GFP localized in the nucleus. The heterologous overexpression of LkWOX4 in 84 K poplar significantly increased AR numbers and decreased AR lengths. In LkWOX4 plants, the endogenous jasmonic acid and abscisic acid contents significantly decreased in stems, while the auxin, jasmonic acid and abscisic acid contents significantly increased in ARs. RNA-Seq of those LkWOX4 overexpression poplar plants showed that the expression of plant hormone signaling genes (ARF2, ARF3, ARF7 and ARF18), rooting-related transcription factors (WOX5, LBD29 and SCR) and root development-related genes (CYCD3, GRF1 and TAA1) were affected. Moreover, we found that LkWOX4 interacts with LkPAT18, LkACBP6, and LkCIP7 using yeast two hybrid screening. Thus, we found LkWOX4 involves in the AR initiation and development, which might be regulated through the IAA, JA and ABA signaling pathways.