文献类型：期刊文献

英文题名：Genome-Wide Identification and Expression Analysis of the KCS Gene Family in Yellow Horn Reveal Their Putative Function on Abiotic Stress Responses and Wax Accumulation

作者：Liu, Xiaojuan[1] Zhao, Ziquan[1] Yang, Yingying[1] Xu, Huihui[1] Bi, Quanxin[1] Wang, Libing[1]

第一作者：刘肖娟

通信作者：Wang, LB[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Res Inst Forestry, Beijing 100091, Peoples R China

年份：2023

卷号：9

期号：1

外文期刊名：HORTICULTURAE

收录：;Scopus(收录号：2-s2.0-85146754812);WOS:【SCI-EXPANDED(收录号:WOS:000917472800001)】；

基金：This work was financially supported by the National Natural Science Foundation of China (31870594, 31901348) and the "Youth Top Talent project" of the "Ten Thousand Talents Program" of the state.

语种：英文

外文关键词：yellow horn; KCS gene; gene expression; stress response; wax

摘要：The beta-ketoacyl CoA synthase encoded by the KCS genes is a rate-limiting enzyme for the synthesis of very-long-chain fatty acid (VLCFA), which catalyzes the VLCFA elongation. Yellow horn (Xanthoceras sorbifolium) is a horticultural tree species known for its kernel oil, which has strong resistance to drought, cold, high temperature, and saline-alkali. The conserved domain FAE1-CUT1-RPPA and ACP-syn-III_C of the KCS gene family were used to search the KCS sequences across the whole genomic sequence of yellow horn; a total of 20 XsKCS genes were identified and divided into four subfamilies. The conserved motif and transmembrane structure analysis revealed that most XsKCSs had a conserved transmembrane domain except XsKCS10 and XsKCS20. The prediction of cis-acting elements of XsKCS genes showed that XsKCS genes contained many stress and hormone response elements, such as ABRE, MBS, and LTR. Furthermore, XsKCS genes exhibited differential expression profiles under abiotic stress and stress-related hormone treatment conditions. Transcriptomic data showed that XsKCS1, XsKCS11, and XsKCS17 had higher expression in yellow horn with high leaf cuticular wax, indicating that they may function in the cuticular wax accumulation and drought response. This study laid a foundation for further functional verification of XsKCS genes in yellow horn stress response.