文献类型：期刊文献

英文题名：Single-nucleotide polymorphisms(SNPs) in a sucrose synthase gene are associated with wood properties in Catalpa fargesii bur

作者：Lu, Nan[1] Mei, Fang[1] Wang, Zhi[1] Wang, Nan[1] Xiao, Yao[1] Kong, Lisheng[2] Qu, Guanzheng[3] Ma, Wenjun[1] Wang, Junhui[1]

第一作者：卢楠

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, State Key Lab Forest Genet & Tree Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Univ Victoria, Dept Biol, Ctr Forest Biol, 3800 Finnerty Rd, Victoria, BC, Canada;[3]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, 26 Hexing Rd, Harbin 150040, Heilongjiang, Peoples R China

年份：2018

卷号：19

期号：1

外文期刊名：BMC GENETICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000449122700001)】；

基金：This work was supported by Forestry Industry Research Special Funds for Public Welfare Projects [201404101]. The funders had no role in study design, data collection and analysis, decision to publish, or the manuscript preparation.

语种：英文

外文关键词：Association study; Single-nucleotide polymorphism; Linkage disequilibrium; Sucrose synthase; Wood properties; Catalpa fargesii bur

摘要：BackgroundAssociation study is a powerful means for identifying molecular markers, such as single-nucleotide polymorphisms (SNPs) associated with important traits in forest trees. Catalpa fargesii Bur is a valuable commercial tree in China and identifying SNPs that associate with wood property would make a foundation of the marker-assisted breeding in the future. However, related work has not been reported yet.ResultsWe cloned a 2887bp long sucrose synthase (SUS) gene from the genome of C. fargesii, which is a key enzyme in sucrose metabolism and also associated to wood formation in trees, coding 806 amino acids that expressed mainly in young branches, xylem, and leaves according to real-time quantitative PCR. Then we identified allelic variations of CfSUS associated with nine wood quality associated traits in Catalpa fargesii Bur. Totally, 135 SNPs were identified through cloning and sequencing the CfSUS locus from a mapping population (including 93 unrelated individuals) and 47 of which were genotyped as common SNPs (minor allele frequency>5%) in the association population that comprised of 125 unrelated individuals collected from main distribution area. Nucleotide diversity and linkage disequilibrium (LD) analysis showed CfSUS has a relative low SNP diversity ((T)=0.0034) and low LD (r(2) dropped below 0.1 within 1600bp). Using the association analysis, we found 11 common SNPs and 14 haplotypes were significantly associated with the traits (false discovery rate, Q<0.1), explaining 3.21-12.41% of the phenotypic variance. These results provide molecular markers above associated with wood basic density, pore rate, and six other traits of wood, which have potential applications in breeding of Catalpa fargesii Bur.ConclusionWe first cloned a SUS gene in C. fargesii, then identified several SNPs and haplotypes that associated with wood properties within this gene, suggesting CfSUS participates in the wood formation of C. fargesii. Moreover, molecular markers we identified in this study may be applied into marker-assisted breeding of C. fargesii in the future.