文献类型：期刊文献

英文题名：Identification and analysis of slow anion channel proteins (SLACs) gene families involved in drought stress in Orchidaceae

作者：Peng, Fu-Cheng[1] Yuan, Meng[1] Hou, Tian-Ze[1] Zhou, Lin[1] Zheng, Bao-Qiang[1] Wang, Yan[1]

第一作者：Peng, Fu-Cheng

通信作者：Wang, Y[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Key Lab Tree Breeding & Cultivat, Natl Forestry & Grassland Adm, Beijing 100091, Peoples R China

年份：2025

卷号：231

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20251918390397);Scopus(收录号：2-s2.0-105004552259);WOS:【SCI-EXPANDED(收录号:WOS:001491229900001)】；

基金：This work was supported by the Basic Research Fund of the Chinese Academy of Forestry, CAF (Grant No. CAFYBB2019ZB001) .

语种：英文

外文关键词：Orchidaceae; Slow anion channel proteins; Functional analysis

摘要：Slow anion channel proteins (SLACs) play a crucial role in stomatal regulation in response to drought stress in plants. Orchids, especially medicinal orchids, have medical and health benefits, and their secondary components (e.g., polysaccharides, alkaloids, etc.) can be used in the production of various industrial products. Drought stress is a key factor limiting the growth of orchid plants, and there is a lack of research on drought stress-related genes in orchid plants. In this study, we identified 40 genes coding for SLAC proteins from the genomes of seven orchid species and analyzed them for basic protein information, structural features, chromosomal localization, promoter elements, collinear analysis, and phylogenetic relationships. We analyzed the expression pattern of the Dendrobium catenatum SLAC gene family. We found that DcaSLAC1 is mainly expressed in leaves and is induced by abscisic acid (ABA), and its encoded protein is localized to the plasma membrane. Functional complementation experiments with the Arabidopsis atslac1 mutant showed that DcaSLAC1 could backfill the stomatal closure behavior of atslac1 that could not be induced by darkness, Ca2 +, and ABA. Heterologous overexpression of DcaSLAC1 in Arabidopsis (Columbia background) enhanced the drought tolerance of the plants. In addition, virus-induced gene silencing (VIGS) of the DcaSLAC1 gene increased stomatal opening rate and stomatal conductance. This study provides important information for further resolving the functions of orchid SLACs and helps to accumulate valuable genetic resources for breeding of D. catenatum for stress tolerance.