文献类型：期刊文献

英文题名：Physiological and gene expression changes of Cryptomeria fortunei Hooibrenk families under heat stress

作者：Xue, Jinyu[1] Zeng, Pingsheng[2] Cui, Jiebing[1] Zhang, Yingting[1] Yang, Junjie[1] Zhu, Lijuan[1] Hu, Hailiang[1] Xu, Jin[1]

第一作者：Xue, Jinyu

通信作者：Xu, J[1]

机构：[1]Nanjing Forestry Univ, Coll Forestry, Coinnovat Ctr Sustainable Forestry Southern China, Key Lab Forest Genet & Biotechnol,Minist Educ, Nanjing, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi, Peoples R China

年份：2023

卷号：14

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85147981681);WOS:【SCI-EXPANDED(收录号:WOS:000932610600001)】；

基金：This work was supported by Fujian Seed Industry Innovation and Industrialization Project of Fujian Province (ZYCX-LY-202101), and Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：Cryptomeria fortunei Hooibrenk; heat stress; physiological analysis; plant cell ultrastructure; gene expression

摘要：Heat stress is one of the major abiotic stresses affecting plant growth and productivity. Cryptomeria fortunei (Chinese cedar) is an excellent timber and landscaping tree species in southern China thanks to its beautiful appearance, straight texture and ability to purify the air and improve the environment. In this study, we first screened 8 excellent C. fortunei families (#12, #21, #37, #38, #45, #46, #48, #54) in a second generation seed orchard. We then analyzed the electrolyte leakage (EL) and lethal temperature at 50% (LT50) values under heat stress, to identify the families with the best heat resistance (#48) and the lowest heat resistance (#45) and determine the physiological and morphological response of different threshold-resistance of C. fortune to heat stress. The relative conductivity of the C. fortunei families showed an increasing trend with increasing temperature, following an "S" curve, and the half-lethal temperature ranges between 39 degrees C and 43.2 degrees C. The activities of SOD and POD fluctuated in the early stage of stress but decreased after 37 degrees C. We observed the changes in the cell ultrastructure at 43 degrees C, and the mesophyll cell structure of #48 was less damaged than that of #45. Eight heat resistance gene, including CfAPX1, CfAPX2, CfHSP11, CfHSP21, CfHSP70, CfHSFA1a, CfHSFB2a and CfHSFB4, were all up-regulated in #45 and #48, and there were significant differences between #45 and #48 under different heat stress treatments. We found a significant difference in heat tolerance between #45 and #48, such that #48 shows higher heat tolerance capability and could be exploited in breeding programs. We conclude that the strongly heat-resistant family had a more stable physiological state and a wider range of heat stress adaptations.