文献类型：期刊文献

英文题名：Global gene expression defines faded whorl specification of double flower domestication in Camellia

作者：Li, Xinlei[1,2] Li, Jiyuan[1,2] Fan, Zhengqi[1,2] Liu, Zhongchi[3,4] Tanaka, Takayuki[5] Yin, Hengfu[1,2]

第一作者：李辛雷

通信作者：Yin, HF[1];Yin, HF[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Key Lab Forest Genet & Breeding, Fuyang 311400, Zhejiang, Peoples R China;[3]Univ Maryland, Dept Cell Biol, College Pk, MD 20742 USA;[4]Univ Maryland, Dept Mol Genet, College Pk, MD 20742 USA;[5]Tokai Univ, Sch Agr, Dept Plant Sci, Kumamoto 8691404, Japan

年份：2017

卷号：7

期号：1

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000402957400078)】；

基金：This work was supported by National Science Foundation of China (NSFC) Grant 31470697, Nonprofit Research Project (CAFYBB2016SZ001) of Chinese Academy of Forestry, and Forestry Industry Research Special Funds for Public Welfare Projects (201504707). We also acknowledge the supports from the Zhejiang province science and technology plan (2013C32075). We thank Drs. N. Ye and T. Yin from Nanjing Forestry University (Nanjing, China) for the help of bioinformatics analysis and sharing computing resources. We are grateful to SL. Xu and WX. Liu for technical assistances in RNA preparation and the gene expression analysis.

语种：英文

摘要：Double flowers in cultivated camellias are divergent in floral patterns which present a rich resource for demonstrating molecular modifications influenced by the human demands. Despite the key principle of ABCE model in whorl specification, the underlying mechanism of fine-tuning double flower formation remains largely unclear. Here a comprehensive comparative transcriptomics interrogation of gene expression among floral organs of wild type and "formal double" and "anemone double" is presented. Through a combination of transcriptome, small RNA and "degradome" sequencing, we studied the regulatory gene expression network underlying the double flower formation. We obtained the differentially expressed genes between whorls in wild and cultivated Camellia. We showed that the formation of double flowers tends to demolish gene expression canalization of key functions; the faded whorl specification mechanism was fundamental under the diverse patterns of double flowers. Furthermore, we identified conserved miRNA-targets regulations in the control of double flowers, and we found that miR172-AP2, miR156-SPLs were critical regulatory nodes contributing to the diversity of double flower forms. This work highlights the hierarchical patterning of global gene expression in floral development, and supports the roles of "faded ABC model" mechanism and miRNA-targets regulations underlying the double flower domestication.