文献类型：期刊文献

英文题名：C2H2 zinc finger protein PagIDD15A regulates secondary wall thickening and lignin biosynthesis in poplar

作者：Pang, Hongying[1] Dai, Xinren[1] Yan, Xiaojing[1] Liu, Yingli[1] Li, Quanzi[1]

第一作者：Pang, Hongying

通信作者：Liu, YL[1];Li, QZ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2024

卷号：346

外文期刊名：PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85196507042);WOS:【SCI-EXPANDED(收录号:WOS:001259589800001)】；

基金：Funding This work was supported by the National Key Research and Devel-opment Program of China (2023YFD2200204) and the Fundamental Research Funds of CAF (CAFYBB2022XC001-1) .

语种：英文

外文关键词：Secondary wall thickening; Lignin; Transcription factor; Poplar

摘要：Wood production is largely determined by the activity of cambial cell proliferation, and the secondary cell wall (SCW) thickening of xylem cells determines the wood property. In this study, we identified an INDETERMINATE DOMAIN (IDD) type C2H2 zinc finger transcription factor PagIDD15A as a regulator of wood formation in Populus alba x Populus glandulosa . Downregulation of PagIDD15A expression by RNA interference (RNAi) inhibited xylem development and xylem cell secondary wall thickening. RNA-seq analysis showed that PagPAL1 , PagCCR2 and PagCCoAOMT1 were downregulated in the differentiating xylem of the PagIDD15A-RNAi transgenic plants, showing that PagIDD15A may regulate SCW biosynthesis through inhibiting lignin biosynthesis. The downregulation of PagVND6-B2 , PagMYB10 and PagMYC4 and upregulation of PagWRKY12 in the differentiating xylem of RNAi transgenic plants suggest that PagIDD15A may also regulate these transcription factor (TF) genes to affect SCW thickening. RT-qPCR analysis in the phloem -cambium of RNAi transgenic demonstrates that PagIDD15A may regulate the expression of the genes associated with cell proliferation, including, PagSHR ( SHORTROOT ), PagSCR ( SCARECROW ), PagCYCD3;1 ( CYCLIN D3;1 ) and PagSMR4 ( SIAMESE-RELATED4 ), to affect the cambial activity. This study provides the knowledge of the IDD-type C2H2 zinc finger protein in regulating wood formation.