文献类型：期刊文献

英文题名：Generation and analysis of expressed sequence tags for microsatellite marker development in Calamus simplicifolius C. F. Wei

作者：Li, Fagen[1,2] Yang, Fan[2] Weng, Qijie[2] Yin, Guangtian[2] Li, Mei[2] Gan, Siming[1,2]

第一作者：李发根

通信作者：Gan, SM[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Guangdong, Peoples R China

年份：2013

卷号：31

期号：4

起止页码：867-877

外文期刊名：MOLECULAR BREEDING

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000317080800011)】；

基金：This work was financially supported by a grant from the Ministry of Science and Technology of China (2011AA100202). The authors wish to thank Dr. Rongsheng Li for kind help in plant sample collection and Changpin Zhou for technical assistance with denaturing polyacrylamide gel electrophoresis as well as Drs. Tom Wood and Jules Freeman for helpful suggestion on an earlier version of the manuscript.

语种：英文

外文关键词：Calamus simplicifolius; Expressed sequence tag (EST); Simple sequence repeat (SSR); Polymorphism

摘要：Rattans serve as an important source of raw non-wood materials for furniture and handicraft industries worldwide. However, their genomic sequence information in public databases is very limited. In this study, a set of 2,528 good-quality expressed sequence tags (ESTs) were generated from a full-length cDNA library constructed previously with root, stem and male inflorescence tissues of Calamus simplicifolius C. F. Wei, a rattan species native to Hainan Island, China. The ESTs were assembled into 1,588 unigenes, including 1,221 singletons and 367 contigs. BlastX searches against the GenBank non-redundant protein database revealed that 1,248 (78.6 %) unigenes had at least one significant match (E a parts per thousand currency sign 10(-5)). The gene ontology functional classification assigned 991, 669 and 977 of the unigenes to the cellular component, molecular function and biological process categories, respectively. A total of 71 simple sequence repeat (SSR) loci were developed among these ESTs, including 65 polymorphic across 19 rattan species representing three genera. High levels of cross-species/genus transferability were observed for the EST-SSRs. For the polymorphic EST-SSR markers, the number of alleles per locus and polymorphic information content ranged from 2 to 25 (mean 11.1) and from 0.135 to 0.949 (mean 0.695), respectively. The EST sequences and the EST-SSR primers have been deposited in GenBank databases of EST (IDs JK838364-40891) and Probe (IDs Pr16718978-9048, to be assigned).