文献类型：期刊文献

英文题名：A GC-MS Protocol for Separating Endangered and Non-endangered Pterocarpus Wood Species

作者：Zhang, Maomao[1,2,3] Zhao, Guangjie[2] Guo, Juan[1,3] Liu, Bo[1,3] Jiang, Xiaomei[1,3] Yin, Yafang[1,3]

第一作者：Zhang, Maomao

通信作者：Yin, YF[1];Yin, YF[2]

机构：[1]Chinese Acad Forestry, Res Inst Wood Ind, Dept Wood Anat & Utilizat, Beijing 100091, Peoples R China;[2]Beijing Forestry Univ, Coll Mat Sci & Technol, Beijing 100083, Peoples R China;[3]Chinese Acad Forestry, Wood Collect WOODPEDIA, Beijing 100091, Peoples R China

年份：2019

卷号：24

期号：4

外文期刊名：MOLECULES

收录：;Scopus(收录号：2-s2.0-85062179596);WOS:【SCI-EXPANDED(收录号:WOS:000460805900145)】；

基金：This work was financially supported by the National High-Level Talent for Special Support Program of China (Grant No. W02020331) and the Fundamental Research Funds of the Chinese Academy of Forestry (Grant No. CAFYBB2017ZE003).

语种：英文

外文关键词：GC-MS; xylariums; orthogonal partial least squares-discriminant analysis (OPLS-DA); species level; wood identification; extract

摘要：Pterocarpus santalinus and Pterocarpus tincorius are commonly used traded timber species of the genus Pterocarpus. P. santalinus has been listed in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES). As a non-CITES species, P. tincorius is also indiscriminately labeled as P. santalinus due to the similar macroscopic and microscopic features with P. santalinus. In order to understand the molecular discrimination between these easily confused species, xylarium heartwoods of these two species were extracted by three different kinds of solvents and analyzed using gas chromatography-mass spectrometry (GC-MS). Multivariate analyses were also applied for the selection of marker compounds that are distinctive between P. santalinus and P. tincorius. A total of twenty volatile compounds were detected and tentatively identified in three kinds of extracts, and these compounds included alcohols, stilbenoids, esters, aromatic hydrocarbons, ketones, miscellaneous, phenols, and flavonoids. GC-MS analyses also revealed that extraction solvents including ethanol and water (EW), ethyl acetate (EA), and benzene-ethanol (BE) gave the best chemotaxonomical discrimination in the chemical components and relative contents of the two Pterocarpus species. After chemometric analyses, EW displayed higher predictive accuracy (100%) than those of EA extract (83.33%) and BE extract (83.33%). Furthermore, spathulenol (17.58 min) and pterostilbene (23.65 min) were elucidated as the critical compounds for the separation of the EW extracts of P. santalinus and P. tinctorius. Thus, a protocol of GC-MS and multivariate analyses was developed to use for successfully distinguishing P. santalinus from P. tinctorius.