文献类型：期刊文献

英文题名：Caldicellulosiruptor saccharolyticus transcriptomes reveal consequences of chemical pretreatment and genetic modification of lignocellulose

作者：Blumer-Schuette, Sara E.[1,3] Zurawski, Jeffrey V.[1] Conway, Jonathan M.[1] Khatibi, Piyum[1] Lewis, Derrick L.[1,4] Li, Quanzi[2,5] Chiang, Vincent L.[2] Kelly, Robert M.[1]

第一作者：Blumer-Schuette, Sara E.

通信作者：Kelly, RM[1]

机构：[1]North Carolina State Univ, Dept Chem & Biomol Engn, Raleigh, NC 27695 USA;[2]North Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA;[3]Oakland Univ, Dept Biol Sci, Rochester, MI USA;[4]Novozymes Biol, Durham, NC USA;[5]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：2017

卷号：10

期号：6

起止页码：1546-1557

外文期刊名：MICROBIAL BIOTECHNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000413904000031)】；

基金：This work was supported by the BioEnergy Science Center (BESC), a U.S. Department of Energy Bioenergy Research Center supported by the Office of Biological and Environmental Research in the DOE Office of Science. JM Conway acknowledges support from a US DoEd GAANN Fellowship (P200A100004-12).

语种：英文

摘要：Recalcitrance of plant biomass is a major barrier for commercially feasible cellulosic biofuel production. Chemical and enzymatic assays have been developed to measure recalcitrance and carbohydrate composition; however, none of these assays can directly report which polysaccharides a candidate microbe will sense during growth on these substrates. Here, we propose using the transcriptomic response of the plant biomass-deconstructing microbe, Caldicellulosiruptor saccharolyticus, as a direct measure of how suitable a sample of plant biomass may be for fermentation based on the bioavailability of polysaccharides. Key genes were identified using the global gene response of the microbe to model plant polysaccharides and various types of unpretreated, chemically pretreated and genetically modified plant biomass. While the majority of C.saccharolyticus genes responding were similar between plant biomasses; subtle differences were discernable, most importantly between chemically pretreated or genetically modified biomass that both exhibit similar levels of solubilization by the microbe. Furthermore, the results here present a new paradigm for assessing plant-microbe interactions that can be deployed as a biological assay to report on the complexity and recalcitrance of plant biomass.