文献类型：期刊文献

中文题名：碳量子点/Fe3+复合材料用于生物传感检测抗坏血酸

英文题名：Carbon dots/Fe3+ composites for biosensor detection of ascorbic acid

作者：高学霞[1] 周希[1,2] 刘美红[1] 马玉峰[2] 王基夫[1,2] 王春鹏[1,2] 储富祥[1,2]

第一作者：高学霞

通信作者：Zhou, Xi|[a0005adfced0a4ac3a671]周希;

机构：[1]中国林业科学研究院林产化学工业研究所,生物质化学利用国家工程实验室,国家林业局林产化学工程重点开放性实验室,江苏省生物质能源与材料重点实验室,江苏南京210042;[2]南京林业大学林业资源高效加工利用协同创新中心,江苏南京210042

年份：2019

卷号：38

期号：7

起止页码：3326-3331

中文期刊名：化工进展

外文期刊名：Chemical Industry and Engineering Progress

收录：CSTPCD;;EI(收录号：20194407615493);Scopus;北大核心:【北大核心2017】；CSCD:【CSCD2019_2020】；

基金：中央级公益性科研院所基本科研业务费专项(CAFYBB2017ZX003)

语种：中文

中文关键词：碳量子点;复合材料;抗坏血酸;生物传感器

外文关键词：carbon dots;composite materials;ascorbic acid;biosensor

分类号：TQ35

摘要：利用碱木质素为原料,在浓硫酸与浓硝酸环境中,先进行超声处理,随后转移至反应釜中,180℃水热反应12h,制备碳量子点(CDs),并采用多种测试手段对其结构及形貌进行了表征。通过CDs溶液与Fe^3+溶液相互作用,制备得到CDs/Fe^3+复合材料。利用紫外光谱及荧光光谱研究了CDs及CDs/Fe^3+复合材料的光学性能,发现CDs具有优异的荧光性能,其荧光量子产率达17.3%(硫酸喹啉为参比物质),而CDs/Fe^3+复合材料荧光猝灭明显。将CDs/Fe^3+复合材料应用于人体内不同生物活性分子检测,发现CDs/Fe^3+复合材料对抗坏血酸(AA)具有特异性识别,且在0~200μmol/L及200~350μmol/L范围内,AA浓度与CDs/Fe^3+复合材料的荧光强度呈现良好的线性关系,同时具有优异的选择识别性,可应用于生物体内AA的检测,在生物传感方面具有潜在的应用价值。
Alkali lignin was used as raw material to prepare carbon dots (CDs) by ultrasonic treatment in concentrated sulfuric acid and nitric acid environment,and transferred to a reactor for hydrothermal reaction at 180℃ for 12h.The structure and morphology of CDs were characterized by various testing methods.CDs/Fe^3+ composites were prepared by the interaction between CDs solution and Fe^3+ solution.The optical properties of CDs and CDs/Fe^3+ composites were studied by ultraviolet and fluorescence spectra.It was found that CDs had excellent fluorescence properties,and the fluorescence quantum yield of CDs was 17.3%(quinoline sulfate was the reference material),while the fluorescence quenching of CDs/Fe^3+ composites was obvious.CDs/Fe^3+ composite materials were used to detect different bioactive molecules in human body.It was found that CDs/Fe^3+ composite materials could specifically recognize ascorbic acid (AA).In the range of 0―200 μmol/L and 200―350 μmol/L,the concentration of AA showed a good linear relationship with the fluorescence intensity of CDs/Fe^3+ composite materials.It could be used in the detection of AA and has potential application in biosensing.