文献类型：期刊文献

英文题名：Alternative Polyadenylation in response to temperature stress contributes to gene regulation in Populus trichocarpa

作者：Yan, Chao[1,2,3,4] Wang, Yupeng[2] Lyu, Tao[1,3] Hu, Zhikang[1,2,3] Ye, Ning[2] Liu, Weixin[3] Li, Jiyuan[3] Yao, Xiaohua[3] Yin, Hengfu[1,3]

第一作者：Yan, Chao

通信作者：Yin, HF[1];Yin, HF[2]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[2]Nanjing Forestry Univ, Coll Informat Sci & Technol, Nanjing, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop, Key Lab Forest Genet & Breeding, Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[4]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Jiangxi, Peoples R China

年份：2021

卷号：22

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000610556200004)】；

基金：This work was supported by Nonprofit Research Projects (CAFYBB2018ZY001-1) of Chinese Academy of Forestry.

语种：英文

外文关键词：Alternative polyadenylation; Abiotic stress; Temperature; Populus

摘要：Background Genome-wide change of polyadenylation (polyA) sites (also known as alternative polyadenylation, APA) is emerging as an important strategy of gene regulation in response to stress in plants. But little is known in woody perennials that are persistently dealing with multiple abiotic stresses. Results Here, we performed a genome-wide profiling of polyadenylation sites under heat and cold treatments in Populus trichocarpa. Through a comprehensive analysis of polyA tail sequences, we identified 25,919 polyA-site clusters (PACs), and revealed 3429 and 3139 genes shifted polyA sites under heat and cold stresses respectively. We found that a small proportion of genes possessed APA that affected the open reading frames; and some shifts were commonly identified. Functional analysis of genes displaying shifted polyA tails suggested that pathways related to RNA metabolism were linked to regulate the APA events under both heat and cold stresses. Interestingly, we found that the heat stress induced a significantly more antisense PACs comparing to cold and control conditions. Furthermore, we showed that a unique cis-element (AAAAAA) was predominately enriched downstream of PACs in P. trichocarpa genes; and this sequence signal was only absent in shifted PACs under the heat condition, indicating a distinct APA mechanism responsive to heat tolerance. Conclusions This work provides a comprehensive picture of global polyadenylation patterns in response to temperatures stresses in trees. We show that the frequent change of polyA tail is a potential mechanism of gene regulation responsive to stress, which are associated with distinctive sequence signatures.