文献类型：期刊文献

英文题名：SPL gene family in Larix kaempferi: identification, functional analysis, and roles in somatic embryogenesis and hormonal regulation

作者：Li, Xin[1,2] Wang, Junchen[2,3,4,5] Huang, Yuqin[2] Li, Chenghao[1] Qi, Liwang[2] Zhang, Lifeng[2]

第一作者：Li, Xin

通信作者：Zhang, LF[1]

机构：[1]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin 150040, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat Natl Forestry, Beijing 100091, Peoples R China;[3]China Three Gorges Corp, Yangtze River Biodivers Res Ctr, Wuhan 430010, Peoples R China;[4]Hubei Key Lab Rare Resource Plants Three Gorges Re, Yichang 443134, Peoples R China;[5]China Three Gorges Corp, Natl Engn Res Ctr Ecoenvironm Protect Yangtze Rive, Wuhan 430014, Peoples R China

年份：2025

卷号：39

期号：5

外文期刊名：TREES-STRUCTURE AND FUNCTION

收录：;EI(收录号：20253719136645);Scopus(收录号：2-s2.0-105015428368);WOS:【SCI-EXPANDED(收录号:WOS:001566871100001)】；

基金：This project was funded by the National Key R&D Program of China (2022YFD2200302), the National Natural Science Foundation of China (31600544 and 32171811), the Biological Breeding-National Science and Technology Major Project (2023ZD0405802).

语种：英文

外文关键词：Larix kaempferi; SPL gene family; Promoter analysis; Somatic embryogenesis; Phytohormone responses

摘要：The SPL gene family plays a crucial regulatory role in plant growth, development, and responses to biotic and abiotic stresses. However, research on the SPL gene family in gymnosperms remains limited. In this study, 12 SPL genes (LkSPLs) from Larix kaempferi were identified and cloned at the whole-genome level and conducted a comprehensive analysis of their sequence characteristics, phylogenetic relationships, conserved domains, cis-elements, and expression patterns. Phylogenetic characterization revealed the segregation of LkSPL proteins into five evolutionarily divergent clades, with constituent members of each cluster maintaining homologous motif organization profiles. Results revealed significant variations in exon-intron structures among LkSPLs, although all members contained conserved SBP domains. Notably, miR156s-binding site was newly identified in LkSPL1. Promoter analysis and hormone response patterns indicated that LkSPLs are transcriptionally regulated by hormones such as salicylic acid (SA), abscisic acid (ABA), indole-3-acetic acid (IAA), gibberellins (GA), and MeJA. qRT-PCR results demonstrated that LkSPL4, LkSPL5, LkSPL6, LkSPL8, LkSPL10, and LkSPL11 were associated with the rapid proliferation and division of proembryogenic masses, while LkSPL1 and LkSPL10 potentially participated in embryonic morphogenesis. Most LkSPLs exhibited minimal transcript levels at 42d during somatic embryo maturation, coinciding with the onset of physiological dormancy. These findings suggest that LkSPLs, as transcription factors, may regulate key processes such as cell division, morphogenesis, maturation, and dormancy during somatic embryogenesis in L. kaempferi, potentially through the miR156/SPL module.