文献类型：期刊文献

中文题名：航天搭载淡紫拟青霉的生物学效应

英文题名：Biological Effect of Space Carrying in Paecilomyces lilacinus

作者：王源[1,2] 汪来发[2] 王曦茁[2] 朱天辉[1]

第一作者：王源

机构：[1]四川农业大学林学院;[2]中国林业科学研究院森林生态环境与保护研究所/国家林业局森林保护学重点实验室

年份：2014

卷号：0

期号：11

起止页码：1933-1940

中文期刊名：核农学报

外文期刊名：Journal of Nuclear Agricultural Sciences

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：国家自然科学基金(31270685);国家自然科学基金青年基金(31100477)

语种：中文

中文关键词：航天诱变;淡紫拟青霉;生物学特征;生物防治

外文关键词：Space mutagenesis;Paecilomyces lilacinus;Biological characteristics;Biological control

分类号：S432.45

摘要：为探讨航天诱变对淡紫拟青霉的生物学效应的影响,利用淡紫拟青霉山东菌株搭载"神舟八号"宇宙飞船进行诱变处理。对筛选得到的30个淡紫拟青霉航天诱变菌株进行了形态、色素、菌丝及孢子形态、菌落生长速度、菌丝干重和产孢量等生物学检测,并测定了各诱变菌株对南方根结线虫卵和松材线虫卵的寄生率。结果表明,淡紫拟青霉航天诱变菌株各项生物学特性与原始菌株存在分化。航天诱变后的菌株菌落形态、色素和菌丝结构与原始菌株差异明显;菌落生长速度、菌丝干重和产孢量的负突变率均高于正突变率;菌株对松材线虫卵的致病力较低,寄生率最高的菌株Sd-m-20的寄生率仅为18.0%,菌株对根结线虫卵致病力较高,菌株Sd-m-9,Sd-m-11,Sd-m-16,Sd-m-22和Sd-m-26对根结线虫卵的寄生率分别提高了14.48%、14.90%、12.35%、7.66%和17.45%,显著高于原始菌株的寄生率(78.3%)。航天处理对淡紫拟青霉诱变效果显著,其优良突变菌株可能用于淡紫拟青霉对南方根结线虫的生物防治。
The biological effect of space mutagenesis on Paecilomyces lilacinus was studied with a P. lilacinus strain Shandong carried by the ‘Shenzhou8 ’spacecraft. 30 strains isolated from the colonies of survival aerospace mutant strains were tested for their biological characteristics The results showed that the biological characteristics of aerospace mutant strains of P. lilacinus were significantly different with the original strain. The morphology,colony growing rate,spore production,dry weight of mycelium and pathogenicity of space mutagenesis strains were significantly different with that of the original strain; growth rate,dry weight and sporular quantity showed higher negative mutation rates; the pathogenicity of the space-induced strains against B. xylophilus eggs was lower,with the highest parasitic rate only18. 0% for Sd-m-20,the space-induced strains to the eggs of M. incognita showed a higher pathogenicity of,with the improved parasitic rates of Sd-m-9,Sd-m-11,Sd-m-16,Sd-m-22 and Sd-m-26 14. 48% 、14. 90%,12. 35%,7. 66%and 17. 45%,respectively,and were higher than the parasitic rate of original strain（ 78. 3%）. The influence of space mutagenesis of P. lilacinus was significant,and the biological characteristics could provide excellent references and a new way in breeding of P. lilacinus superior strain. Space mutagenesis could be used as to obtain new nematophagous fungal strains for biological control of M. incognita.