文献类型：期刊文献

英文题名：Characterization of walnut JrWOX11 and its overexpression provide insights into adventitious root formation and development and abiotic stress tolerance

作者：Chang, Yingying[1,2] Song, Xiaobo[2] Li, Mingjun[1] Zhang, Qixiang[3] Zhang, Pu[2] Lei, Xiashuo[2] Pei, Dong[2]

第一作者：Chang, Yingying

通信作者：Pei, D[1]

机构：[1]Henan Normal Univ, Coll Life Sci, Engn Technol Res Ctr Nursing & Utilizat Genuine Ch, Engn Lab Green Med Mat Biotechnol Henan Prov, Xinxiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry, Beijing, Peoples R China;[3]Zhejiang A&F Univ, Sch Forestry & Biotechnol, Nurturing Stn State Key Lab Subtrop Silviculture, Linan, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000860294900001)】；

基金：This research was supported by the National Natural Science Foundation of China (Grant No. 32101479), the State Key Laboratory of Tree Genetics and Breeding program (Grant No. CAFYBB2019ZY001), and the Postdoctoral Initiative Foundation of Henan Normal University (Grant No. 5101049470215).

语种：英文

外文关键词：JrWOX11; overexpression; adventitious root formation; abiotic stress tolerance; motif-"GGAIQY

摘要：The well-developed root system enables plant survival under various environmental stresses. WUSCHEL-RELATED HOMEOBOX GENE 11 (WOX11) plays a critical role in adventitious root formation and development in rice, Arabidopsis, and easy-to-root tree poplar. However, in difficult-to-root trees, the knowledge of WOX11 during adventitious root formation and development remains scarce. In this study, the JrWOX11 gene was isolated from a difficult-to-root tree walnut and heterologously expressed in the "84K" poplar. The results showed that JrWOX11 contained a similar structure and sequence to the homologous genes in rice, Arabidopsis, and poplar, but had different numbers and types of motifs and cis-elements. JrWOX11 lacked the motif GGAIQY compared to that in easy-to-root trees. In addition, JrWOX11 expression was induced by ABA, PEG, and NaCl treatments. Overexpression of JrWOX11 in poplar promoted root initiation and significantly increased adventitious root (ARs) number, lateral roots (LRs) number, and root hair (RH) length. Furthermore, the aboveground biomass was notably increased under NaCl and PEG treatments in transgenic plants. When NaCl and PEG were removed, the survival rate, aerial shoot development, and de novo root organogenesis were also markedly enhanced in transgenic shoot cuttings. The study provides valuable information on the differences between JrWOX11 and the homologous genes in rice, Arabidopsis, and poplar, and supports the critical role of JrWOX11 in the formation of AR and tolerance to salt and osmotic stresses.