文献类型：期刊文献

英文题名：Comprehensive analysis of AP2/ERF gene members in Acer truncatum B. and the positive regulator role of AtruDREB28 in drought tolerance

作者：Li, Jianbo[1,2] Guo, Wei[3] Meng, Huijing[1] Zhao, Jinna[1] Zheng, Guanshun[1] Yuan, Weijie[1]

第一作者：李建波

通信作者：Yuan, WJ[1]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, China Natl Permanent Sci Res Base Warm Temperate Z, Beijing 102300, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Taishan Acad Forestry Sci, Tai An 271000, Peoples R China

年份：2023

卷号：200

外文期刊名：INDUSTRIAL CROPS AND PRODUCTS

收录：;EI(收录号：20231914061452);Scopus(收录号：2-s2.0-85156249006);WOS:【SCI-EXPANDED(收录号:WOS:001004567100001)】；

基金：This work was supported by the National Nonprofit Institute Research Grant of the Chinese Academy of Forestry (CAFYBB2021QB006 and CAFYBB2022QC003) .

语种：英文

外文关键词：AP2; ERF family; AtruDREB28; Expression analysis; Acer truncatum; Drought stress

摘要：APETALA2/Ethylene Responsive Factor (AP2/ERF) play pivotal roles in resisting diverse stresses and in regulating growth and development in plant, but the information and function of the AP2/ERF genes are not well elucidated in Acer truncatum B. As an important woody tree producing oil with high economic values, A. truncatum has strong tolerance to drought. AP2/ERF may implicate in drought stress tolerance and growth of A. truncatum. In the current research, the AtruAP2/ERF family members were identified and comprehensive bioinformatics analysis were performed, including phylogenetic relationship, gene/protein structure, expression pattern, cis- acting element, protein interaction network, and functional characterization of AtruDREB28. Total of 117 AtruAP2/ERF members were identified in A. truncatum genome and were divided into five subfamilies, AP2 (17 genes), ERF (61 genes), DREB (35 genes), RAV (3 genes), and Soloist (one gene). The members from same clade exhibited relatively similar gene structure and motif compositions. Fifty-seven duplication events were identified in AtruAP2/ERF family, containing eight pairs generated from fragmental duplication, and 49 pairs generated from tandem duplication. Subsequently, a variety of cis-acting elements associated with development, hormone, and stress were identified in the promoter regions of AtruAP2/ERF, such as ARE, MBS, ABRE, TCA, etc. A po-tential interaction network of AtruAP2/ERFs was conducted to predict the interaction relationships. In addition, tissue expression analysis showed that majority of AtruAP2/ERFs exhibited tissue specific modes. The expression profiles of the majority of AtruAP2/ERFs were induced in response to drought stress, suggesting that AtruAP2/ ERFs were involved in the modulation of drought stress response. Furthermore, overexpression of AtruDREB28 increased the tolerance to drought stress by enhancing the reactive oxygen species-scavenging capability. Together, these results give insights into the characteristics and functional analysis of AtruAP2/ERF genes in A. truncatum.