文献类型：期刊文献

英文题名：Metabolomic and Confocal Laser Scanning Microscopy (CLSM) Analyses Reveal the Important Function of Flavonoids in Amygdalus pedunculata Pall Leaves With Temporal Changes

作者：He, Yueyue[1] Pan, Lei[1] Yang, Tao[2,3] Wang, Wei[4] Li, Cong[1] Chen, Bang[1] Shen, Yehua[1]

第一作者：He, Yueyue

通信作者：Li, C[1];Shen, YH[1]

机构：[1]Northwest Univ, Coll Chem & Mat Sci, Natl Demonstrat Ctr Expt Chem Educ, Key Lab Synthet & Nat Funct Mol Chem,Minist Educ, Xian, Peoples R China;[2]Shaanxi Acad Forestry, Xian, Peoples R China;[3]Technol Res Ctr Amygdalus Pedunculata State Fores, Yulin, Peoples R China;[4]Chinese Acad Forestry, Inst Forestry, State Forestry Adm, Key Lab Silviculture, Beijing, Peoples R China

年份：2021

卷号：12

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;Scopus(收录号：2-s2.0-85107213893);WOS:【SCI-EXPANDED(收录号:WOS:000656855000001)】；

基金：We were very grateful for the financial support from the Amygdalus pedunculata Engineering Technology Research Center of the State Forestry and Grassland Administration and Key Laboratory of Yulin Desert Plant Resources.

语种：英文

外文关键词：Amygdalus pedunculata Pall leaves; metabolomics; flavonoids; UPLC-QTOF-MS; confocal laser scanning microscopy

摘要：Amygdalus pedunculata Pall [Rosaceae, Prunus, Prunus pedunculata (Pall.) Maxim.] belongs to the Rosaceae family and is resistant to cold and drought. Ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry and metabolomics were used to track the changes in bioactive metabolites during several stages of Amygdalus pedunculata Pall growth. A total of 827 different metabolites were detected, including 169 flavonoids, 68 organic acids, 35 terpenoids and 2 tannins. Flavonoid biosynthesis and flavone and flavonol biosynthesis were the main synthetic sources of flavonoids. Quercetin, isoquercitrin, and epicatechin as biomarkers related to growth and development were found. Quercetin connects the biosynthesis of flavonoids and the biosynthesis of flavones and flavonols. The contents of isoquercitrin and epicatechin increased uniformly during the whole growth process from the flowering stage to the fruit ripening stage, indicating that play key roles in the fruit growth and ripening stages of this plant. The tissue location and quantitative analysis of flavonoids in leaves at different stages were performed by confocal laser scanning microscopy. The flavonoids were mainly distributed in the palisade tissue and spongy tissue, indicating the need for protection of these sensitive tissues in particular. Through comprehensive and systematic analysis, the temporal distribution of flavonoids in the process of their leaves growth was determined. These results clarify the important role of flavonoids in the developmental process of Amygdalus pedunculata Pall.