文献类型：期刊文献

英文题名：In vitro amphidiploid induction of a distant hybrid Populus simonii × P. euphratica cv. 'Xiaohuyang-2' and its effect on plant morphology and anatomy

作者：Zhang, Xiao-Xiao[1,2,3,4] Zhang, Ying[1,2,3,4] Cui, Xiao-Tong[1,2,3,4] Li, Dai-Li[5] Zhang, Heng-Yue[5] Duan, Wula[6] Wang, Jun[1,2,3,4]

第一作者：Zhang, Xiao-Xiao

通信作者：Wang, J.[1,2,3,4]

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Beijing Forestry University, Beijing, 100083, China; [2] National Engineering Research Center of Tree Breeding and Ecological Restoration, Beijing Forestry University, Beijing, 100083, China; [3] Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, MOE, Beijing Forestry University, Beijing, 100083, China; [4] College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China; [5] Beijing Institute of Landscape Architecture, Beijing, 100102, China; [6] Experimental Center of Desert Forestry, Chinese Academy of Forestry, Dengkou County,,,Inner Mongolia, 015200, China

年份：2022

卷号：71

期号：1

起止页码：107-115

外文期刊名：Silvae Genetica

收录：EI(收录号：20230613545951)

语种：英文

外文关键词：Agronomy - Chromosomes - Morphology

摘要：Highly gametic sterility of a distant hybrid Populus simonii × P. euphratica cv. 'Xiaohuyang-2' restricts its utilization in breeding programs of Populus. Amphidiploid induction by somatic chromosome doubling is expected to restore its gametic fertility. In this study, nodal-segment and leaf explants of 'Xiaohuyang-2' were used to induce chromosome doubling with colchicine in vitro. Although chromosome doubling of the nodal-segment explants only produced mixoploids, the treatments of leaf explants on adventitious bud regeneration medium successfully produced 4 amphidiploids, which might be attributed to the direct organogenesis of the adventitious buds on the leaf explants. This is the first report of amphidiploid induction in a distant hybrid between Populus section Tacamahaca and sect. Turanga. The highest amphidiploid induction frequency was 16.7 %. Both the explant survival rate and polyploidization frequency were significantly affected by colchicine concentration and exposure time. The amphidiploid plants significantly differed from the diploid and mixoploid plants in morphological and anatomical characteristics. They had larger, thicker, and greener leaves than the diploids and mixoploids. The increase in ploidy level also resulted in changes in stomatal features. The induced amphidiploid plants of the distant hybrid 'Xiaohuyang-2' are expected to play important roles in breeding programs of Populus in the future, which can be used as a bridge parent with the ability of unreduced gamete formation to cross with fast-growth germplasms to produce triploids pyramiding desirable traits of fast growth, easy cutting propagation, and salt and drought tolerances. ? 2022 Xiao-Xiao Zhang et al., published by Sciendo.