文献类型：期刊文献

中文题名：基于聚焦离子束-扫描电子显微技术的雪松木质部具缘纹孔三维重构

英文题名：The three-dimensional structure of bordered pit in xylem of Cedrus deodara base on focused ion beam scanning electron microscopy

作者：王静[1] 王杰[2] 郭娟[2] 冯韵[3] 李喜霞[3] 张建国[3] 姜笑梅[2] 殷亚方[2] 李姗[1,2]

第一作者：王静

机构：[1]陕西科技大学,环境科学与工程学院,陕西西安710021;[2]中国林业科学研究院木材工业研究所,北京100091;[3]中国科学院生物物理所生物成像中心,北京100101

年份：2022

卷号：41

期号：1

起止页码：66-71

中文期刊名：电子显微学报

外文期刊名：Journal of Chinese Electron Microscopy Society

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：国家林业和草原局林草青拔项目(No.2020132601);国家自然科学基金青年基金资助项目(No.32001291);陕西科技大学“青年拔尖”人才支持项目(No.126022037).

语种：中文

中文关键词：雪松;具缘纹孔;木质部;三维重构

外文关键词：Cedrus deodara;bordered pits;xylem embolism;three-dimensional reconstruction

分类号：Q944.5;S718.47

摘要：木本植物木质部具缘纹孔是木质部水分输导、栓塞形成、扩散及疲劳产生的关键部位,同时也是影响木材干燥与防腐等加工工艺的重要构造特征。目前,针对具缘纹孔结构的成像技术大多集中在二维尺度,难以精确地反映其三维结构的相关信息。本文利用聚焦离子束-扫描电子显微镜,通过对雪松木质部管胞具缘纹孔进行连续切片和图像采集,并采用Imaris软件重构其三维结构,分辨率达5 nm。聚焦离子束-扫描电子显微技术实现了具缘纹孔三维结构的精准成像,能够反映具缘纹孔膜三维孔隙结构,有助于从微纳尺度揭示木质部水分输导、栓塞形成、扩散及疲劳的机制,并为木材干燥和保护提供科学依据。
Bordered pits in plant xylem are the key locations where xylem embolism forms, spreads and xylem fatigue occurs, and are also important microstructures affecting thedrying and preserving processes of the wood. At present, the imaging techniques of bordered pit structures are mostly concentrated in the two-dimensional scale, which could hardly reflect accurately the relevant information of the three-dimensional structure of bordered pits. In this paper, we adopted the focused ion beam scanning electron microscopy(FIB-SEM) to cut serial sections of bordered pits in xylem of Cedrus deodara, moreover, we reconstructed the three-dimensional structures of bordered pits using Imaris software with a maximum resolution of 5 nm. The focused ion beam scanning electron microscopy technique achieves accurate imaging of the three-dimensional structure of bordered pit and can reflect the three-dimensional structure of bordered pits membrane, which helps to reveal the mechanism of xylem embolism formation, spread and fatigue from the micro-nano scale and provides theoretical reference materials for wood drying and protection.