文献类型：期刊文献

英文题名：Amphipathic antibacterial agents using cationic methacrylic polymers with natural rosin as pendant group

作者：Chen, Ying[1,2,3] Wilbon, Perry A.[2,3] Chen, Yung Pin[2,3] Zhou, Juhua[4] Nagarkatti, Mitzi[4] Wang, Chunpeng[1] Chu, Fuxiang[1] Decho, Alan W.[2,3] Tang, Chuanbing[2,3]

第一作者：陈莹;Chen, Ying

通信作者：Chen, Y[1]

机构：[1]Chinese Acad Forestry, Inst Chem Ind Forestry Prod, Nanjing 210042, Jiangsu, Peoples R China;[2]Univ S Carolina, Dept Environm Hlth Sci, Dept Chem & Biochem, Columbia, SC 29208 USA;[3]Univ S Carolina, USC Nanoctr, Columbia, SC 29208 USA;[4]Univ S Carolina, Dept Pathol Microbiol & Immunol, Sch Med, Columbia, SC 29209 USA

年份：2012

卷号：2

期号：27

起止页码：10275-10282

外文期刊名：RSC ADVANCES

收录：;EI(收录号：20124215577200);Scopus(收录号：2-s2.0-84867391510);WOS:【SCI-EXPANDED(收录号:WOS:000312138400025)】；

基金：This work was supported by China International Science and Technology Cooperation (2011DFA32440), Forestry Public Sector Research Fund of State Forestry Administration of China (CAFYBB2010003-3), the University of South Carolina (start-up funds), and USDA NIFA (Award 2011-51160-31205) and National Science Foundation (DMR-1206072).

语种：英文

外文关键词：Chlorine compounds - Escherichia coli - Hydrophobicity - Natural polymers - Scanning electron microscopy

摘要：We prepared a class of novel cationic polymers as antimicrobial agents: quaternary ammonium-containing poly(N,N-dimethylaminoethyl methacrylate) with natural rosin as the pendant group (PDMAEMA-g-rosin). Different from most other amphipathic antimicrobial polymeric systems reported in the literature, our approach sandwiched the hydrophilic cationic group between the polymer backbone and bulky hydrophobic hydrophenanthrene side groups. A simple quaternization reaction was used to link the rosin ester chloride and PDMAEMA homopolymers. Both the Gram-positive bacterium Staphylococcus aureus (S. aureus) and Gram-negative bacterium Escherichia coli (E. coli) were tested against the PDMAEMA-g-rosin copolymers. PDMAEMA-g-rosin copolymers with the amphipathic structure exhibited effective antimicrobial activity against both E. coli and S. aureus. Both the degree of quaternization of rosin group and the molecular weight of PDMAEMA played roles in antimicrobial activities. Our results also indicated that conformation of hydrophobic group (particularly steric hindrance) played a role in dictating antibacterial efficacy. Scanning electron microscopy and confocal laser scanning microscopy were used to characterize morphological changes of bacteria after exposure with PDMAEMA-g-rosin copolymers. Possible mechanisms on a combination of ionic and hydrophobic interactions between bacterial cells and polymers are discussed.