文献类型：期刊文献

中文题名：油橄榄Ⅲ类过氧化物酶家族的进化和表达分析

英文题名：Evolution and expression analysis of the classⅢperoxidase family in olive

作者：薛丽[1] 刘晓霞[1] 王晨合[1] 张建国[1,2,3] 饶国栋[1,2,3]

第一作者：薛丽

机构：[1]林木遗传育种国家重点实验室,中国林业科学研究院林业研究所,北京100091;[2]南京林业大学南方现代林业协同创新中心,江苏南京210037;[3]国家林业和草原局林木培育重点实验室,中国林业科学研究院林业研究所,北京100091

年份：2023

卷号：45

期号：4

起止页码：36-49

中文期刊名：北京林业大学学报

外文期刊名：Journal of Beijing Forestry University

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：中央级公益性科研院所专项(CAFYBB2021QC001)。

语种：中文

中文关键词：油橄榄;Ⅲ类PRXs;复制基因;新功能;非生物胁迫

外文关键词：Olea europaea;classⅢperoxidase;replicator;new function;abiotic stress

分类号：S718.46;S565.7

摘要：【目的】Ⅲ类过氧化物酶(PRX)是一类植物特有的氧化还原酶,在植物生长发育和胁迫响应方面具有十分重要的作用。本研究拟探讨油橄榄Ⅲ类PRX基因家族的进化和表达模式,旨在为油橄榄分子育种提供参考。【方法】利用生物信息学方法鉴定并分析了油橄榄Ⅲ类PRX基因家族的系统发育关系、分组、染色体分布、复制基因、基序分布、基因结构图、顺式作用元件分布和在不同组织和不同非生物胁迫下的表达,并对部分成员基因进行了实时荧光定量PCR验证。【结果】(1)鉴定得到了106个OePRX基因,根据其与拟南芥和毛果杨PRX蛋白序列的系统进化关系分为了14个组。(2)OePRX基因不均匀地分布于23条染色体上;片段复制是该基因家族扩张的主要动力,且复制基因在进化过程中受到了较强的纯化选择;与拟南芥相比,油橄榄PRX基因与毛果杨PRX基因的亲缘关系更近。(3)同一组中OePRX蛋白的等电点、分子量、基序分布、基因结构、信号肽分布、在不同组织中的表达模式均比较保守;OePRX基因启动子中含有较多的生长发育元件和激素应答元件;在油橄榄干热胁迫和水涝胁迫下,38%的OePRX基因显著差异表达。【结论】油橄榄OePRX基因家族明显的进化扩张和多变的表达模式暗示了其功能的复杂性,尤其是复制基因的新功能化对油橄榄在地中海地区的广泛分布具有十分重要的适应性意义。
[Objective]The classⅢperoxidase is a group of plant-specific oxidoreductases,and plays an important role in plant development and stress response.This paper presents a detailed overview of evolution and expression of olive classⅢPRX gene family,aiming to provide a reference for olive molecular breeding in the future.[Method]With some bioinformatic tools,we finished the identification of the olive classⅢPRX genes,completed the analyses of phylogenetic relationship,gene mapping on the chromosomes,pairs of duplicates,motifs,gene structure,cis-acting elements and gene expression in different tissues or biotic stresses,and did a verification of RNA-seq by RT-qPCR.[Result](1)106 OePRX genes were obtained,and classified into 14 groups based on the phylogeny with AtPRX and PtPRX.(2)OePRX genes were unevenly located on 23 chromosomes,and ragment replication was the main driving force for the expansion of gene family.Compared with AtPRX,OePRX had a closer relationship with PtPRX.(3)The characterization of pI,MW,motifs,gene structure,signal peptide,and expression in different tissues had the expected group-conserved patterns.The promoters of OePRX contained a variety of development and harmone elements;38%of OePRX genes was differentially expressed in heat,drought and waterlogged stress.[Conclusion]The differential expansion and differential expression patterns may imply flexibility in neofunctionalization of duplicated classⅢperoxidase genes,which is of adaptive significance to the strong resistance of olive to a diversity of conditions,hence contributing to the importance of olive as a Mediterranean Basin staple food.