文献类型：期刊文献

中文题名：檀香组培苗瓶外微扦插研究

英文题名：Ex vitro rooting of Santalum album microshoots by micro-cutting

作者：刘英[1]

第一作者：刘英

机构：[1]中国林业科学研究院热带林业研究所,广东广州510520

年份：2022

卷号：42

期号：5

起止页码：74-82

中文期刊名：中南林业科技大学学报

外文期刊名：Journal of Central South University of Forestry & Technology

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：广东省林业科技创新项目(2017KJCX025)。

语种：中文

中文关键词：檀香;预生根培养;微扦插;基质配比

外文关键词：Santalum album;pre-rooting culture;micro-cutting;medium composition

分类号：S722.8

摘要：【目的】开展檀香组培苗瓶外微扦插研究,旨在解决组培苗规模生产的关键问题,推动檀香优良无性系的推广应用。【方法】以1号无性系为试材,通过糖质量浓度以及NH_(4)NO_(3)与KNO_(3)质量浓度的对比试验,分析瓶苗的生长状况,确定预生根培养基;通过基质配比和预生根苗高的对比试验,分析基质和苗高对微扦插生根率及根系状态的影响,确定适宜的微扦插基质配比和预生根苗高。进一步以1、12、18、19、21、22号等6个优良无性系为材料,验证预生根培养基效果,对比分析6个无性系的微扦插生根难易程度。【结果】蔗糖质量浓度以及NH_(4)NO_(3)与KNO_(3)质量浓度对檀香瓶苗生长和落叶影响显著(P<0.05);瓶苗高度和落叶率随着蔗糖质量浓度的升高分别呈现先增后降和降低的趋势,随着NH_(4)NO_(3)质量浓度的增加呈现增大和先降后升的趋势,随着KNO_(3)质量浓度的增加呈先升后降或先稳定后升高的趋势;采用40 g·L^(-1)蔗糖、660 mg·L^(-1) NH_(4)NO_(3)及1520 mg·L^(-1) KNO_(3)开展预生根培养,其瓶苗生长旺盛且不落叶,为适宜的预生根培养基。基质和瓶苗高度显著影响檀香微扦插生根率和根系发育;微扦插生根率随黄心土比例增加、蛭石减少呈现先上升后下降的趋势;应用蛭石、泥炭土和黄心土混合基质(6∶1∶3,V/V),微扦插生根成活率最高(68.08%),为檀香微扦插的适宜基质。微扦插生根率随着瓶苗高度的增大亦呈先升后降的趋势,以4.6~5.0 cm高瓶苗的生根效果最好。不同无性系之间,以18号无性系微扦插生根率最高(87.12%),其次是21号(70.79%)及1号(68.23%)无性系。【结论】通过组培生产预生根瓶苗,再进行瓶外微扦插的檀香无性系苗木培育方案是可行的。建议生产上采用18号、21号和1号优良无性系予以推广,以解决檀香良种壮苗的规模化供应问题。
【Objective】Sandalwood is a kind of famous wood in the world,which rooting is difficult during the process of tissue culture.This study aimed to solve this key issue for the large-scale production of tissue culture plantlets and to promote the application of elite sandalwood clones.【Method】Taking the No.1 clone as the material,through the comparison test of sugar,NH_(4)NO_(3) and KHNO_(3) concentrations,the suitable medium for the pre-rooting culture was determined on the basis of the growth performance analysis of plantlets.Through the comparison test of matrix ratio and the height of pre-rooted seedlings,the effect of different media and plantlet height on the rooting percentage and root properties was investigated,and the suitable medium and plantlet height were screened for rooting.Furthermore,six clones(No.1,No.12,No.18,No.19,No.21,and No.22)were used to confirm the effect of the pre-rooting medium,and the rooting difficulties of the six clones were compared.【Result】The concentrations of sucrose,NH_(4)NO_(3) and KNO_(3) influenced the growth and defoliation of sandalwood plantlets significantly(P<0.05).With the increase of sucrose concentration,the height of the seedlings increased first and then decreased,while the defoliation rate showed a declining tendency.With the increase of NH_(4)NO_(3) concentration,the height increased monotonously,while the defoliation rate declined first and then increased.As the KNO_(3) concentration increased,the height of the seedlings increased first and then declined,while the defoliation rate stabilized first and then increased with the KNO_(3) concentration.The medium containing 40 g·L^(-1) sucrose,660 mg·L^(-1) NH_(4)NO_(3) and 1520 mg·L^(-1) KNO_(3) was the most suitable pre-rooting culture for sandalwood plantlets.The seedlings grew well without defoliation.The medium composition and plantlet height significantly affected the rooting rate and root development.The rooting rate increased first and then decreased with the increase of soil proportion and the decrease of vermiculite proportion.The mixed medium of vermiculite,peat and soil(6∶1∶3,V/V)was selected as the best ex vitro rooting medium with a survival rate of plantlets up to 68.08%.The plantlets with a height of 3.6-5.5 cm were suitable for ex vitro micro-cutting of sandalwood,while the plantlets with a height of 4.6-5.0 cm performed the best.Among the six clones,No.18 showed the highest rooting rate which was up to 87.19%,followed by No.21(70.79%)and No.1(68.23%).【Conclusion】In conclusion,it is feasible to produce the pre-rooted plantlets of sandalwood by tissue culture,and then micro-cut plantlets ex vitro.It is suggested that the clones No.18,No.21 and No.1 should be applied in the large-scale production of sandalwood plantlets,in order to solve the problem of mass supply of elite sandalwood clones.