文献类型：期刊文献

英文题名：Transcriptome Analysis of Populus Overexpression in SVL Transcription Factor

作者：Wang, Dongli[1,2] Cheng, Renwu[3] Liu, Yunshan[2] Wang, Shengkun[2] Yang, Zhende[1] Meng, Sen[2]

第一作者：Wang, Dongli

通信作者：Yang, ZD[1]

机构：[1]Guangxi Univ, Coll Forestry, Guangxi Coll & Univ Key Lab Forestry Sci & Engn, Nanning 530004, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Guangzhou 510520, Peoples R China;[3]Guangzhou Inst Forestry & Landscape Architecture, Guangzhou 510520, Peoples R China

年份：2023

卷号：14

期号：9

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001082743200001)】；

基金：We thank Xihang Ai for his help in reviewing and correcting the references.

语种：英文

外文关键词：Populus; transcriptome analysis; overexpression; transcription factor

摘要：Flowering is an essential part of the productive process, and flowering time is determined by endogenous genetic components and many ambient factors. SHORT VEGETATIVE PHASE (SVP), a MADS-box transcription factor, regulates floral transition by repressing floral integrator genes and is involved in ABA-mediated drought stress. In this study, we transformed the poplar (Populus) clone "84K" with the SVP-Like gene, while stable overexpression transgenic lines were obtained. Transcriptome analysis of the leaves of the transgenic lines and WT (Wide Type) poplars revealed that a total of 477 genes showed significantly altered expression, overexpressing SVL genes, including 342 upregulated and 135 downregulated genes. Ten subclusters in DEGs were analyzed, and KEGG terms of the largest subcluster were associated with two key pathways: hormone-related genes and glutathione metabolism. Meanwhile, many transcriptional factors were involved. Our results are helpful for in-depth analysis of the MADS transcriptional factor in poplars. This work provides the basis for studying woody plant growth, and development and molecular mechanisms responded to environmental stresses.