文献类型：期刊文献

英文题名：Genome-wide transcriptomic analysis of a desert willow, Salix psammophila, reveals the function of hub genes SpMDP1 and SpWRKY33 in drought tolerance

作者：Jia, Huixia[1] Zhang, Jin[1,2] Li, Jianbo[3] Sun, Pei[1] Zhang, Yahong[1] Xin, Xuebing[3] Lu, Mengzhu[1] Hu, Jianjun[1]

第一作者：Jia, Huixia

通信作者：Zhang, J[1];Hu, JJ[1]

机构：[1]Chinese Acad Forestry, Natl Forestry & Grassland Adm, Key Lab Tree Breeding & Cultivat, Res Inst Forestry,State Key Lab Tree Genet & Bree, Beijing 100091, Peoples R China;[2]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA;[3]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China

年份：2019

卷号：19

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85070951723);WOS:【SCI-EXPANDED(收录号:WOS:000481846800002)】；

基金：This work was supported by National Nonprofit Institute Research Grant of CAF (CAFYBB2017ZY008), (CAFYBB2018ZY001-9), and Natural Science Foundation of China (31800570). The founders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Salix psammophila; Drought; Co-expression network; Hub gene; Transgene; SpMDP1; SpWRKY33

摘要：Background Drought is a major environmental constraint to plant growth, development and productivity. Compared with most willows that are generally susceptible to drought, the desert willow Salix psammophila has extraordinary adaptation to drought stress. However, its molecular basis of drought tolerance is still largely unknown. Results During polyethylene glycol 6000 (PEG 6000)-simulated drought stress, we found that the osmotic adjustment substances were accumulated and the antioxidant enzyme activities were enhanced in S. psammophila roots. A total of 8172 differentially expressed genes were identified in roots of S. psammophila through RNA-Sequencing. Based on K-means clustering, their expression patterns were classified into nine clusters, which were enriched in several stress-related processes including transcriptional regulation, response to various stresses, cell death, etc. Moreover, 672 transcription factors from 45 gene families were differentially expressed under drought stress. Furthermore, a weighted gene co-expression network was constructed, and eight genes were identified as hub genes. We demonstrated the function of two hub genes, magnesium-dependent phosphatase 1 (SpMDP1) and SpWRKY33, through overexpression in Arabidopsis thaliana. Overexpression of the two hub genes enhanced the drought tolerance in transgenic plants, suggesting that the identification of candidate drought tolerance genes in this study was highly efficient and credible. Conclusions Our study analyzed the physiological and molecular responses to drought stress in S. psammophila, and these results contribute to dissect the mechanism of drought tolerance of S. psammophila and facilitate identification of critical genes involved in drought tolerance for willow breeding.