文献类型：期刊文献

英文题名：Functional characterization of an unobtrusive protein, CkMT4, in re-establishing desiccation tolerance in germinating seeds

作者：Peng, Long[1,3] Wu, Hao[1] Huang, Xu[1] Zeng, Meiyan[1] Deng, Shiming[1,2] Xue, Hua[1] Wang, Xiaofeng[1]

第一作者：彭龙;Peng, Long

通信作者：Xue, H[1];Wang, XF[1]

机构：[1]Beijing Forestry Univ, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Natl Engn Lab Tree Breeding, Coll Biol Sci & Biotechnol, 35 Tsinghua East Rd, Beijing 100083, Peoples R China;[2]Hubei Minzu Univ, Coll Forestry & Hort, Enshi, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Peoples R China

年份：2021

卷号：173

起止页码：180-192

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;Scopus(收录号：2-s2.0-85099710656);WOS:【SCI-EXPANDED(收录号:WOS:000623235400018)】；

基金：This work was supported by the National Key Research and Development Project of China (2017YFE0105100), the National Natural Science Foundation of China (31971646), the Fundamental Research Funds for the Central Universities (2019ZY26), the Fundamental Research Funds for the Central Non-profit Research of Chinese Academy of Forestry (CAFYBB2020QB002), the National Natural Science Foundation of China (31901290) and the National Training Program of Innovation and Entrepreneurship for Undergraduates (201810022036).

语种：英文

外文关键词：Desiccation tolerance; Metallothionein; Caragana korshinskii; Antioxidant; Seed

摘要：Desiccation tolerance (DT) is gradually lost during seed germination, while it can be re-established by pretreatment with polyethylene glycol (PEG) and/or abscisic acid (ABA). Increasing knowledge is available on several stress-related proteins in DT re-establishment in herb seeds, but limited information exists on novel proteins in wood seeds. This study aimed to investigate the role of metallothionein CkMT4, a protein species with the highest fold increase in abundance in Caragana korshinskii seeds on PEG treatment. The fluctuation in mRNA levels of CkMT4 during seed development was consistent with the changes in DT, and the expression of CkMT4 could be up-regulated by ABA. Besides metal-binding capacity, CkMT4 might supply Cu2+/Zn2+ to superoxide dismutase (SOD) under high redox potential provided by PEG treatment for excess reactive oxygen species (ROS) scavenging. The overexpression of CkMT4 in yeast results in enhanced oxidation resistance. Experimentally, this study demonstrated the overexpression of CkMT4 in Arabidopsis seeds benefited the reestablishment of DT and enhanced the activity of SOD. On the whole, these findings suggested that CkMT4 facilitated the re-establishment of DT in C. korshinskii seeds mainly through diminishing excess ROS, which put the mechanism underlying the re-establishment of DT in xerophytic wood seeds into a new perspective. (C) 2021 Elsevier B.V. All rights reserved.