文献类型：期刊文献

英文题名：SpHsfA4c from Sedum plumbizincicola Enhances Cd Tolerance by the AsA-GSH Pathway in Transgenic Populus x canescens

作者：Yu, Miao[1,2] He, Zhengquan[3] Li, Shaocui[1] Lu, Zhuchou[1] Chen, Juanjuan[1] Qu, Tongbao[4] Xu, Jing[1] Qiu, Wenmin[1] Han, Xiaojiao[1] Zhuo, Renying[1]

第一作者：Yu, Miao

通信作者：Han, XJ[1];Zhuo, RY[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Peoples R China;[2]Nanjing Forestry Univ, Forestry Fac, Nanjing 210037, Peoples R China;[3]China Three Gorges Univ, Biotechnol Res Ctr, Key Lab Three Gorges Reg Plant Genet & Germplasm E, Yichang 443002, Peoples R China;[4]Jilin Agr Univ, Coll Forestry & Grassland, Changchun 130118, Peoples R China

年份：2023

卷号：13

期号：3

外文期刊名：AGRONOMY-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000953815400001)】；

基金：This work was supported by National Natural Science Foundation of China, (No. 31872168) and National Key Research and Development Program of China (No. 2021YFD2200201).

语种：英文

外文关键词：SpHsfA4c; Cd tolerance; AsA-GSH metabolism pathway; transgenic poplars

摘要：The ascorbate (AsA)-glutathione (GSH) metabolism pathway is an important antioxidant system in cadmium (Cd) detoxification; the AsA-GSHpathway is generally regulated by a specific set of functional genes. However, transcription factors involved in AsA-GSH pathway have yet to be identified. Herein, we transformed a heat shock transcription factor SpHsfA4c from Sedum plumbizincicola into Populus. x canescens. Under 100 mu M CdCl2 stress for 30 d, the leaf chlorosis of wild-type poplars (WT) is more serious than that in transgenic poplars. The root biomass, shoot biomass and tolerance index (TIs) of transgenic poplars were higher than those in WT. In addition, transgenic poplars have higher Cd2+ uptake and Cd content. Compared with WT, the contents of hydrogen peroxide (H2O2) and superoxide anion (O-2(center dot-)) in transgenic poplars were significantly reduced in leaves under Cd treatment. The expression levels of five enzymes (ascorbate peroxidase (APX), catalases (CAT), superoxide dismutase (SOD), peroxidase (POD) and glutathione S-transferase (GST)) were higher in transgenic poplars than those in WT. Transgenic poplars contained higher concentrations of intermediate metabolites, including GSH, AsA and phytochelatins (PCs), and a higher GSH/GSSG ratio in the AsA-GSH metabolism pathway. In Fourier transform infrared (FTIR) spectra, the characteristic peaks indicated that the contents of cysteine, GSH and AsA in transgenic poplars were exceeded compared to those in WT. These results suggested that SpHsfA4c can activate the AsA-GSH metabolism pathway to reduce Cd-associated oxidative stress. Therefore, overexpressing SpHsfA4c in P. x canescens can give rise to a superior Cd tolerance. Our results provide a theoretical significance for breeding potential new germplasm resources with high biomass and high Cd tolerance for remediation of soil heavy metal pollution.