文献类型：期刊文献

英文题名：PeSHN1 regulates water-use efficiency and drought tolerance by modulating wax biosynthesis in poplar

作者：Meng, Sen[1,2] Cao, Yang[3] Li, Huiguang[1] Bian, Zhan[4] Wang, Dongli[1] Lian, Conglong[1] Yin, Weilun[1] Xia, Xinli[1]

第一作者：孟森;Meng, Sen

通信作者：Xia, XL[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Guangzhou 510520, Guangdong, Peoples R China;[3]Northwest A&F Univ, Inst Soil & Water Conservat, State Key Lab Soil Eros & Dryland Farming Loess P, Yangling 712100, Shaanxi, Peoples R China;[4]Peking Univ, Coll Life Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

年份：2019

卷号：39

期号：8

起止页码：1371-1386

外文期刊名：TREE PHYSIOLOGY

收录：;Scopus(收录号：2-s2.0-85071703120);WOS:【SCI-EXPANDED(收录号:WOS:000493055600009)】；

基金：This research was supported by grants from the National Natural Science Foundation of China (31770649, 31570308 and 31670610) and China Postdoctoral Science Foundation (2017M620024 and 2018T110056).

语种：英文

外文关键词：drought tolerance; PeSHN1; Populus; water-use efficiency; wax biosynthesis

摘要：Wax, a hydrophobic structure that provides an effective waterproof barrier to the leaves, is an important drought adaptation trait for preventing water loss. However, limited knowledge exists regarding the molecular mechanisms underlying wax biosynthesis in trees. Here, PeSHN1, an AP2/ethylene response factor transcription factor, was isolated from a fast-growing poplar Populus x euramericana cv. 'Neva' clone. To study the potential biological functions of PeSHN1, transgenic 84K poplar (Populus alba x Populus glandulosa) plants overexpressing PeSHN1 were generated. PeSHN1 overexpression resulted in decreased transpiration, increased water-use efficiency (WUE) and increased drought tolerance. The transgenic poplar plants exhibited increased wax accumulation and altered wax composition, mainly because of a substantial increase in long-chain (>C30) fatty acids, aldehydes and alkanes. Gene expression analyses revealed that many genes involved in wax biosynthesis were induced in the PeSHN1 overexpression plants. In addition, chromatin immunoprecipitation-PCR assays and dual luciferase assays revealed that at least one of those genes, LACS2, is likely targeted by PeSHN1. Moreover, the PeSHN1 overexpression plants maintained higher photosynthetic activity and accumulated more biomass under drought stress conditions. Taken together, these results suggest that PeSHN1 regulates both WUE and drought tolerance in poplar by modulating wax biosynthesis and that altered PeSHN1 expression could represent a novel approach (altering the wax trait on leaf surfaces to increase WUE) for breeding drought-tolerant plants.