文献类型：期刊文献

中文题名：濒危植物扇脉杓兰野生居群遗传多样性的AFLP分析

英文题名：Genetic diversity of endangered wild Cypripedium japonicum populations: An AFLP analysis

作者：钱鑫[1] 李全健[1,2] 连静静[1] 王彩霞[1] 田敏[1]

第一作者：钱鑫

机构：[1]中国林业科学研究院亚热带林业研究所;[2]中国科学院上海辰山植物科学研究中心

年份：2013

卷号：32

期号：6

起止页码：1445-1450

中文期刊名：生态学杂志

外文期刊名：Chinese Journal of Ecology

收录：CSTPCD;;Scopus;北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：浙江省重大科技攻关项目(2010C02004-2)资助

语种：中文

中文关键词：扇脉杓兰;AFLP;遗传结构;遗传距离;基因流;保育策略

外文关键词：Cypripedium japonicum; AFLP; genetic structure; genetic distance; gene flow; conversation strategy

分类号：Q75

摘要：扇脉杓兰(Cypripedium japonicum)是一种珍稀的地生兰。为了探索并制定针对性的保护措施,采用AFLP分子标记技术对现存于浙江省的6个扇脉杓兰天然居群进行了遗传多样性和遗传结构的研究。筛选出的7对引物共扩增出377条清晰条带,多态性比率为20.95%。居群总Nei基因多样性指数为0.0512,其中居群内基因多样性为0.0247,居群平均Shannon多态性信息指数为0.0417,群体间总遗传分化系数为0.5178,基因流为0.4655,表明扇脉杓兰居群的遗传多样性较低,居群间出现一定程度的遗传分化。UPGMA聚类和Mantel检测分析结果表明,居群间的地理位置与遗传距离之间不存在显著相关性(r=0.16,P=0.242)。基于本研究结果并结合扇脉杓兰的生物学特性,初步提出了相应的保育策略。
Cypripedium japonicum is a rare and endangered terrestrial orchid. To explore and for- mulate the pertinent conversation strategies of this species, this paper analyzed the genetic diver- sity and genetic structure of six natural C. japonicum populations in Zhejiang Province of East China by the method of AFLP. A total of 377 distinct bands were amplified by 7 pairs of selected primers. The percentage of polymorphic loci was 20.95%, Nei＇ s genetic diversity index was 0. 0512, Shannon information index was 0. 0417, genetic differentiation coefficient was 0.5178, and gene flow was 0. 4655, indicating that there was a relatively low genetic diversity within the C. japonicum populations, and a certain degree of genetic divergence existed among the popula- tions. The analyses of UPGMA clustering and Mantel test showed that there was no significant correlation between the geographical distance and genetic distance （ r = 0.16, P = 0.242 ）. Some conservation strategies were proposed based on this study and the biological properties of C. japonicum.