文献类型：期刊文献

英文题名：Methyl Jasmonate Orchestrates Multi-Pathway Antioxidant Defense to Enhance Salt Stress Tolerance in Walnut (Juglans regia L.)

作者：Nie, Ruining[1,2,3] Wu, Chengxu[1,2,3] Ji, Xinying[1,2,3] Li, Ao[1,2,3] Zheng, Xu[1,2,3] Tang, Jiajia[4] Sun, Leyuan[1,2,3] Su, Yi[1,2,3] Zhang, Junpei[1,2,3]

第一作者：Nie, Ruining

通信作者：Zhang, JP[1];Zhang, JP[2];Zhang, JP[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[2]State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Key Lab Tree Breeding & Cultivat State Forestry &, Beijing 100091, Peoples R China;[4]Chongqing Acad Forestry, Chongqing 400036, Peoples R China

年份：2025

卷号：14

期号：8

外文期刊名：ANTIOXIDANTS

收录：;Scopus(收录号：2-s2.0-105014523488);WOS:【SCI-EXPANDED(收录号:WOS:001557198000001)】；

基金：This work was supported by the National Key Research and Development Program of China (Grant Number: 2022YFD2200402).

语种：英文

外文关键词：Juglans regia L.; MeJA; NaCl; phenylalanine pathway; physiological indices; transcriptome

摘要：Walnut (Juglans regia L.), an ecologically and economically important species, requires the elucidation of its salt stress response mechanisms for improved salt tolerance breeding. This study elucidates the physiological and molecular mechanisms through which exogenous methyl jasmonate (MeJA) mitigates salt stress in walnut, providing novel strategies for salt-tolerant cultivar development. This integrated study combined physiological, biochemical, and multi-omics analyses to decipher how exogenous MeJA enhances ROS scavenging through the synergistic activation of phenylalanine (Phe), tryptophan (Trp), and alpha-linolenic acid pathways, establishing a multilevel antioxidant defense network. MeJA treatment effectively mitigated salt stress-induced oxidative damage, as demonstrated by a significant 16.83% reduction in malondialdehyde (MDA) content, concurrent 11.60%, 10.73% and 22.25% increases in superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT) activities, respectively, the elevation of osmoregulatory soluble sugars (SS), and 1.2- to 2.0-fold upregulation of key antioxidant enzyme genes (SOD, POD, APX, GPX, DHAR) and elevated osmoregulatory substances (soluble sugars, SS). Improved photosynthetic parameters (Pn, Gs) and chlorophyll fluorescence efficiency (Fv/Fm) collectively indicated reduced oxidative stress (improved by 7.97-23.71%). Joint metabolomic-transcriptomic analyses revealed MeJA-enhanced ROS scavenging via the coordinated regulation of Phe, Trp, and alpha-linolenic acid pathways. In summary, MeJA significantly enhanced reactive oxygen species (ROS) scavenging efficiency and comprehensive antioxidant capacity in walnut seedlings through the synergistic regulation of key metabolic pathways, effectively mitigating salt stress. These findings establish a crucial mechanistic foundation for understanding plant salt stress responses and advance the utilization of MeJA-mediated strategies for the genetic improvement of salinity tolerance in walnut. Future research should prioritize optimizing MeJA application protocols and functionally validating key regulatory genes for breeding applications.