文献类型：期刊文献

中文题名：基因芯片及其在环境微生物研究中的应用

英文题名：Microarrays and Their Application to Environmental Microorganisms

作者：张于光[1,2] 李迪强[1] 肖启明[3] 刘学端[3]

第一作者：张于光

机构：[1]中国林业科学研究院森林生态环境与保护研究所;[2]湖南农业大学生物安全科技学院长沙410128;[3]湖南农业大学生物安全科技学院

年份：2004

卷号：44

期号：3

起止页码：406-410

中文期刊名：微生物学报

外文期刊名：Acta Microbiologica Sinica

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：基因芯片;环境微生物;群落结构;功能基因

外文关键词：Microarray, Environmental microorganism, Community structure, Functional gene

分类号：Q343.1

摘要：基因芯片因其具有高密度、高灵敏度、快速 (实时 )检测、经济、自动化和低背景水平等特点 ,而广泛应用于不同的研究领域。目前 ,应用于环境微生物研究的基因芯片主要有功能基因芯片 (FGAs)、系统发育的寡核苷酸芯片 (POAs)和群落基因组芯片 (CGAs)。综述了基因芯片在环境微生物研究中的应用 ,包括自然环境中微生物的基因表达分析、比较基因组分析和混合微生物群落的分析等。
Microarray take advantage of the advanced technologies of molecular biology, semi-conduct microelectronics, laser, chemical dyes and so on, and is the high-cross and comprehensive advanced science and hot spot of study. Compared with traditional nucleic acid methods, microarray offer the additional advantages of high density, high sensitivity, rapid (real-time) detection, lower cost, automation, and low background levels and so on. Therefore, microarray-based technology has been used in many fields and is potentially well suited for identifying populations of microorganisms in natural environments. The microarray used in environmental microbial studies can be divided into three major classes, including functional gene arrays(FGAs), phylogenetic oligonucleotide arrays(POAs) and community genome arrays(CGAs). In this review, we viewed microarray's application to research on environmental microorganisms, such as analyzing microbial gene express in environment, comparable genome analysis and environmental microbial community analysis. At last, the challenges and future perspectives of microarray in natural environment was discussed.