文献类型：期刊文献

中文题名：高温对川硬皮肿腿蜂发育及Wolbachia携带的影响

英文题名：Effects of High Temperature on the Progeny Development and Wolbachia Infection of Sclerodermus sichuanensis(Hymenoptera:Bethylidae)

作者：唐艳龙[1] 康奎[1] 陈思琪[1] 王丽娜[1] 车华涛[1] 王小艺[2] 魏可[2]

第一作者：唐艳龙

机构：[1]遵义师范学院生物与农业科技学院/贵州省赤水河流域动物资源保护与应用研究重点实验室,遵义563002;[2]中国林业科学研究院森林生态环境与自然保护研究所/国家林业和草原局森林保护学重点实验室,北京100091

年份：2023

卷号：39

期号：4

起止页码：752-759

中文期刊名：中国生物防治学报

外文期刊名：Chinese Journal of Biological Control

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD2023_2024】；

基金：国家自然科学基金(31901319,31700573);国家级大学生创新创业训练计划项目(202010664010);赤水河流域动物资源保护与应用研究特色重点实验室建设项目(黔教合KY字-2013-111)。

语种：中文

中文关键词：川硬皮肿腿蜂;温度;发育历期;子代数量;沃尔巴克氏体

外文关键词：Sclerodermus sichuanensis;temperature;developmental duration;offspring number;Wolbachia

分类号：S476.3

摘要：温度是影响昆虫生长发育最重要的因子,也是影响昆虫共生微生物活性的重要因素。为了明确高温对川硬皮肿腿蜂Sclerodermus sichuanensis的生殖和发育特征及对其体内Wolbachia携带率的影响,本研究在27℃、30℃和33℃温度条件下连续繁育川硬皮肿腿蜂4代,测定不同温度下肿腿蜂的寄生能力、产卵前期、子代历期、子代数量和雄性比的差异;同时以Wolbachia特异的外膜蛋白基因(wsp)进行PCR扩增,检测是否携带该菌。结果显示,3个温度对川硬皮肿腿蜂的寄生率和子代羽化率均无显著影响,但对其产卵前期和子代发育有显著影响。随温度升高,雌蜂产卵前期延长,但子代的卵历期、幼虫历期和蛹历期显著缩短。在27℃、30℃和33℃下,子代卵历期分别为4.33 d、3.27 d和3.36 d;幼虫历期分别为6.77 d、5.85 d和5.07 d;蛹历期分别为13.85 d、12.35 d和10.96 d。在33℃下,幼期发育耗时较27℃时缩短了约5 d。川硬皮肿腿蜂单雌子代数随温度的变化无显著差异,但产雄数和子代雄性比例随温度升高显著增加,33℃下的单雌产雄数和雄性比例较27℃时提升了近3倍。连续繁育4代后川硬皮肿腿蜂Wolbachia携带率存在显著差异,27℃下肿腿蜂Wolbachia携带率为100%,但在33℃下其携带率下降至6.67%,温度与携带率存在显著的负相关关系。以上结果表明,在27~33℃,温度升高可显著缩短川硬皮肿腿蜂的繁育周期,增加子代雄性比例,证实了高温具有显著抑制该虫体内的Wolbachia活性从而增加子代雄性比的功能。繁育温度过高对川硬皮肿腿蜂生殖适合度是不利的,规模化繁育温度不宜超过30℃。
Temperature is a most important factor affecting insect development and the activity of insect symbiotic microbial.In order to explore the effects of high temperature on the reproduction,developmental traits,and Wolbachia infection of the parasitic wasp,Sclerodermus sichuanensis,the parasitoids reared under three temperatures(27,30,and 33℃)for four consecutive generations were observed for the parasitism ability and pre-oviposition period of female,progeny developmental duration,offspring number,and male proportion.Meanwhile,the Wolbachia infection of the female parasitoids was detected using the specific primers of the outer surface protein gene(wsp).The results showed that high temperature had no significant effect on the females'parasitism ability,but significantly influenced on their pro-oviposition period and progeny development.With the increase of temperature,the pre-oviposition period of females was prolonged,but the durations of immature stage were significantly shortened.The duration of egg stage was 4.33,3.27,and 3.36 d;duration of larval stage was 6.77,5.85,5.07 d;duration of pupal stage was 13.85,12.35,10.96 d in the three temperature treatments,respectively.The durations of parasitoid immature stage at 33℃were shortened by approximately 5 d in comparison to parasitoids rearing at 27℃.There were no significant differences in the offspring numbers at different temperatures,but the male numbers and proportions exhibited significant increase with the increasing temperatures.Male numbers and proportions of parasitoids reared at 33℃were approximately three times larger than those at 27℃.Additionally,all the parasitoid individuals were infected by Wolbachia after four generations of breeding at 27℃;however,Wolbachia infection rate of female parasitoids decreased to 6.67%at 33℃.There is a significant negative correlation between temperature and Wolbachia infection rate.Our findings indicate that high temperature shortens the developmental durations of the parasitoids,but also results in the reproductive fitness loss attributed to the significantly increased of male proportions.Our results also confirm that the activity of Wolbachia is inhibited at high temperature,and thus accordingly increases the proportion of the male offspring.The temperature of artificial mass-rearing of the parasitoid,S.sichuanensis,should not exceed 30℃.