文献类型：期刊文献

中文题名：苹果小吉丁虫微卫星开发及其种群遗传结构分析

英文题名：Microsatellite development and genetic diversity analysis of apple buprestid Agrilus mali Matsumura

作者：房冰[1] 段辛乐[1] 张彦龙[2] 杨忠岐[2] 陈茂华[1]

第一作者：房冰

机构：[1]西北农林科技大学植物保护学院,农业部西北黄土高原作物有害生物综合治理重点实验室,陕西杨凌712100;[2]中国林业科学研究院,森林生态环境与保护研究所,北京100091

年份：2017

卷号：44

期号：4

起止页码：559-566

中文期刊名：植物保护学报

外文期刊名：Journal of Plant Protection

收录：CSTPCD;;北大核心:【北大核心2014】；CSCD:【CSCD2017_2018】；

基金：国家公益性行业(林业)科研专项(201404403)

语种：中文

中文关键词：苹果小吉丁虫;微卫星;磁珠;富集文库;遗传结构

外文关键词：Agrilus mali Matsumura; microsatellite; magnetic-bead; enriched library; genetic structure

分类号：S436.611.2

摘要：为研究苹果小吉丁虫Agrilus mali Matsumura的种群遗传结构,采用磁珠富集法以生物素标记探针(AC)_(12)和(AG)_(12)构建苹果小吉丁虫微卫星文库,根据阳性克隆测序获得的微卫星侧翼序列设计引物,筛选和开发苹果小吉丁虫多态性微卫星标记,并利用开发的微卫星标记对其不同地理种群的遗传结构进行分析。结果显示,从微卫星文库中的300个阳性克隆中筛选得到248个含有微卫星片段的克隆子,阳性克隆率为82.7%,其中完美型微卫星131个,占52.8%;不完美型90个,占36.3%;混合型27个,占10.9%,表明磁珠富集法开发新微卫星标记的效率较高。从获得的248个苹果小吉丁虫微卫星中筛选获得7个多态性微卫星位点,这7个位点在苹果小吉丁虫8个地理种群样本中的等位基因数为10~23个,有效等位基因数为1.674~12.218个,多态信息含量为0.304~0.796。8个苹果小吉丁虫种群的观测杂合度为0.095~0.676,期望杂合度为0.469~0.755,Shannon指数为0.791~1.621,遗传距离为0.071~0.788。采自新疆维吾尔自治区的7个苹果小吉丁虫种群之间遗传相似度较高,但其与辽宁省种群的遗传相似度较低。
To research population genetics of apple buprestid Agrilus mali Matsumura, a microsatellite- enriched genomic library of A. mall was constructed by using the magnetic-bead enrichment method. Bi- otinylated （AC）12 and （AG）12 probes were used to screen the microsatellite sequences of the pest. For screening and developing the polymorphic microsatellite loci, primers were designed based on the conserved flanking sequences of the microsatellites acquired from the sequences of the positive clones. The results showed that 248 of the 300 randomly picked positive clones contained microsatellites, with a screening efficiency of 82.7%. Among the 248 microsatellite locus sequences, 131 loci （accounting for 52.8%） consisted of perfect repeat motifs, 90 loci （36.3%） contained imperfect repeat motifs, and 27 loci （10.9%） had compound repeat motifs, indicating that the magnetic-bead enrichment method was efficient to develop new microsatellites ofA. mali. Seven polymorphic microsatellite loci screened from the 248 microsatellites showed 10-23 alleles in A. mali samples from eight geographical regions. The effective number of alleles （Ne）, the polymorphic information content （PIC）, the observed heterozygosity （Ho）, the expected heterozygosity （He）, the Shannon index and the genetic distance in those samples were 1.674-12.218, 0.304-0.796, 0.095-0.676, 0.469-0.755, 0.791-1.621 and 0.071-0.788, respective- ly. The genetic similarity between populations from Xinjiang Uygur Autonomous Region was high and those between populations from Xinjiang Uygur Autonomous Region and Liaoning Province were low.