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赤霉素和生长素浸种对野牛草种子萌发及幼苗生长生理的影响     被引量:39

Seed soaking in exogenous gibberellin and auxin and their impact on seed germination and seedling physiology of Buchloe dactyloides(Nutt.)Engelm.

文献类型:期刊文献

中文题名:赤霉素和生长素浸种对野牛草种子萌发及幼苗生长生理的影响

英文题名:Seed soaking in exogenous gibberellin and auxin and their impact on seed germination and seedling physiology of Buchloe dactyloides(Nutt.)Engelm.

作者:邹竣竹[1] 韩蕾[1] 李德颖[2] 孙振元[1]

第一作者:邹竣竹

机构:[1]中国林业科学研究院林业研究所/国家林业局林木培育重点实验室;[2]美国北达科他州立大学种植学院

年份:2017

卷号:34

期号:9

起止页码:1838-1846

中文期刊名:草业科学

外文期刊名:Pratacultural Science

收录:CSTPCD;;北大核心:【北大核心2014】;CSCD:【CSCD2017_2018】;

基金:国家863项目重要草坪草优质抗逆分子育种技术研究与新品种创制(2011AA100209)

语种:中文

中文关键词:野牛草;赤霉素;生长素;幼苗生长;碳水化合物;内源激素

外文关键词:buffalograss; auxin; gibberellin;germination; seedling growth; carbohydrate; endogenous hormone

分类号:S543.903;Q945.34

摘要:为研究外源激素对野牛草(Buchloe dactyloides)种子破除休眠和幼苗生长生理的作用,本研究分别设置3个浓度的赤霉素(GA3)和生长素(IAA)对野牛草进行浸种处理,浸种后进行发芽试验,对浸种后种子的萌发及幼苗生长情况、非结构性碳水化合物和内源激素进行测定。结果表明,1)赤霉素浸种可以有效增强野牛草种子发芽力,提高发芽势、发芽率、发芽指数和种子活力指数,其中,2 000mg·L-1的赤霉素处理效果最显著(P<0.05);适当浓度的生长素浸种可以有效增强野牛草种子的生活力。2)赤霉素处理有助于野牛草幼苗地上部的生长;生长素处理对野牛草幼苗地下部和根冠比有显著(P<0.05)的增强作用,其中较低浓度(250mg·L-1)处理效果最明显。3)1 500mg·L-1赤霉素与450mg·L-1生长素浸种均可显著(P<0.05)提高野牛草幼苗可溶性糖与淀粉的积累。4)较高浓度的外源赤霉素和生长素浸种处理会使野牛草幼苗内源生长素、赤霉素、脱落酸(ABA)含量上升,其中,2 000 mg·L-1的赤霉素和450mg·L-1的生长素浸种效果最佳。综上所述,打破野牛草种子休眠、促进地上部生长及其幼苗内源赤霉素含量的最佳试剂和浓度为2 000 mg·L-1的赤霉素,增加可溶性糖和淀粉在野牛草幼苗期积累的最佳试剂和浓度是1 500mg·L-1的赤霉素;促进野牛草幼苗地下部生长的最佳试剂和浓度是250mg·L-1的生长素,增加野牛草幼苗内源生长素和脱落酸含量的最佳试剂和浓度是450mg·L-1的生长素。
In order to study the effects of exogenous hormones on breaking seed dormancy, and seedling growth of buffalo grass, seeds were soaked in three concentrations of gibberellic acid(GAa) and auxin(IAA) separate- ly. After soaking, seed germination, seedling growth, non-structural carbohydrates, and endogenous hormone levels were measured. Our results showed: 1) Seed germination GA3 increased germination vigor and increased germination, germination rate, germination index and seed vigour index. Based on the treatment effects, gib- berellin at 2 000 mg ·L^-1 had the most significant impact. Auxin also effectively enhanced the viability of the seeds, of which 450 mg ·L^-1 auxin yielded the best results. 2) Seedling growth GAa treatment promoted the growth of aerial parts. IAA effectively increased buffalo grass seedling root length and low concentration of 250 mg·L^-1, which yielded the best results. 3) Seedling non-st optimalGA3 concentration was 1 500 ·L^-1 while forIAAit was 450 mg·L^-1, wh crease the content of soluble sugars and starch in buffalo grass seedlings. 4) Seedling root to bud ratio, at a ructural carbohydrates- ich could effectively in- endogenous hormones buffalo grass seedlings and shoot growth is IAA, when applied at a concentration of 250 mg ·L^-1. Auxin at a concentration of 450 mg ·L^-1 was found to be the best hormone to increase endogenous IAA and ABA levels in buffalo grass.

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