文献类型：期刊文献

中文题名：欧洲黑杨育种基因资源SSR多态性比较研究

英文题名：Comporison of Genetic Variations of Black Poplar (Populus nigra L.) Gene Resource by Microsatellite Markers

作者：张香华[1] 苏晓华[1] 黄秦军[1] 张冰玉[1]

第一作者：张香华

机构：[1]中国林业科学研究院林业研究所国家林业局林木培育重点实验室

年份：2006

卷号：19

期号：4

起止页码：477-483

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：农业科技成果转化资金项目:"西丰杨系列无性系生产性试验"(05EFN216700382);"948"项目:"北方型美洲黑杨纸浆材基因资源及分子育种技术引进"(2005-4-51)

语种：中文

中文关键词：欧洲黑杨;基因资源;SSR;遗传多样性

外文关键词：Populus nigra; gene resource; SSR; genetic diversity

分类号：S792.11

摘要：本研究利用13对SSR引物对120份（105份国外引进、15份我国新疆）欧洲黑杨基因资源进行了遗传多态性分析，共检测出171个等位基因，每个多态性位点检测到7～19个等位基因，平均为13．2，多态信息指数为0．396～0．909，平均为0．808；遗传距离为0～0．4564。欧洲黑杨基因种源具有丰富的遗传多样性。通过UPGMA等类分析，将120份材料划分为8类，地理分布较近的材料基本聚在一起，表明遗传距离与地理距离相关性强。
The simple sequence repeat （SSR） or microsatellite marker is currently preferred in genetic analysis due to its highly desirable properties. 13 pairs of SSR primers were used to detect genetic diversity among 120 Populus nigra L. gene resource. A total of 171 allels were observed among the pelymorphic loci, 7～19 SSR alleles were detected for each locus with an average of 13.2 alleles per locus. 13 pairs of SSR primers were polymorphic with the pelymorphism information content （PIC） ranging from 0.396 to 0.909. The 120 clones were clustered based on the banding patterns generated by 13 polymorphic SSR loci. In general, this study indicates that SSRs are sufficiently abundant and polymorphic to be useful genetic markers in genetic diversity study of Populus nigra L. gene resources. This study provided theoretic basis for efficient utilization of these gene resources.