文献类型：期刊文献

英文题名：Characterization and putative post-translational regulation of alpha- and beta-tubulin gene families in Salix arbutifolia

作者：Rao, Guodong[1,2,3] Zeng, Yanfei[1,3] He, Caiyun[1,3] Zhang, Jianguo[1,2,3]

第一作者：饶国栋;Rao, Guodong

通信作者：Zhang, JG[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing 210037, Jiangsu, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China

年份：2016

卷号：6

外文期刊名：SCIENTIFIC REPORTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000368108600002)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (31400569), Fundamental Research Funds for the Central Non-profit of CAF (CAFYBB2014QB028), Collaborative Innovation Plan of Jiangsu Higher Education (2013-2015), the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (RIF2013-11), Beijing Co-building Plan for Scientific Research and Postgraduate Education(2013K0140, 2013K0141, 2014K0151, 2014K0152), we thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

语种：英文

摘要：Microtubules, which are composed of heterodimers of alpha-tubulin (TUA) and beta-tubulin (TUB) proteins, are closely associated with cellulose microfibril deposition and play pivotal roles in plant secondary cell wall development. In the present study, we identified eight TUA and twenty TUB genes in willow (Salix arbutifolia). Quantitative real-time PCR analysis showed that the small number of TUA gene family members relative to that of TUBs was complemented by a higher transcript copy number for each TUA gene, which is essential to the maintenance of the tubulin 1:1 heterodimer assembly. In Salix, five of eight TUAs were determined to be unusual because these contained a C-terminal methionine acid, leucine acid, glutamic acid, and glutamine acid, instead of the more typical tyrosine residue, which in turn generated the hypothesis of post-translational modifications (PTMs) that included deleucylation, demethiolation, deglutamynation, and deaspartylation. These PTMs are responsible for the removal of additional amino acid residues from TUAs prior to detyrosination, which is the first step of C-terminal PTMs. The additional PTMs of the TUA gene family might be responsible for the formation of different tubulin heterodimers that may have diverse functions for the adaptation of the woody perennial growth for Salix.