文献类型：期刊文献

中文题名：毛白杨α-微管蛋白基因家族的克隆与序列分析

英文题名：Cloning and Sequences Analysis of α-tubulin Genes Family in Populus tomentosa

作者：饶国栋[1,2] 睢金凯[1] 张建国[1,2]

第一作者：饶国栋

机构：[1]南京林业大学南方现代林业协同创新中心,江苏南京210037;[2]国家林业局林木培育重点实验室中国林业科学研究院林业研究所,北京100091

年份：2015

卷号：25

期号：1

起止页码：44-49

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2014】；CSCD:【CSCD2015_2016】；

基金：中央级公益性科研院所基本科研业务费专项资金项目(RIf2013-11);国家自然科学基金青年项目(31400569);江苏高校协同创新计划资助项目

语种：中文

中文关键词：α-微管蛋白;生物信息学;系统进化;三维结构模拟

外文关键词：α-tubulin; bioinformatics; phylogenetic analysis; three-dimensional simulation

分类号：S718.6

摘要：采用本地化软件Blast P对杨树全基因组数据库和Gen Bank数据进行搜索,根据搜索结果对毛白杨TUA基因家族进行克隆,并利用软件Clustal W和MEGA对TUA基因进行了核酸和氨基酸序列的比对分析和系统进化分析;利用SWISS-MODEL服务器对TUA的蛋白质三维结构进行模拟。结果显示:毛白杨α-微管蛋白由8个成员构成,8个成员的氨基酸序列和其它植物α-微管蛋白序列的相似性在80%以上;TUA基因可分为2个家族,每个家族成员都有其独特的内含子外显子特征;TUA蛋白的三维结构表现出极高的相似性。毛白杨TUA基因在序列和结构上都十分保守;8个TUA基因起源于2个祖先TUA基因。
In order to study the bioinformatics characteristics of Populus tomentosa Carr. , the local software Blast P was used to search the TUA gene members of Populus in the database of Populus whole genome and Genbank and then the TUA genes family was cloned. By using the software Clustal W and MEGA, the sequence alignment and phylogenetic analysis of TUA genes were conducted. Finally, the TUA protein simulation structures were gotten by the SWISS-MODEL. The result showed that the P. tomentosa α-tubulin genes family consisted of 8 TUA genes mem- bers. The 8 members can be divided into two classes, and each class had its own exon-intron property. The three- dimensional structures of the 8 TUA proteins showed extreme similarity. It is proved that the TUA genes have conserve gene and protein structure, and 8 Populus TUA genes are originated from one ancestor.