文献类型：期刊文献

英文题名：Anticancer activity of a novel glycoprotein from Camellia oleifera Abel seeds against hepatic carcinoma in vitro and in vivo

作者：Li, Tingting[1,2] Meng, Xiangyong[3] Wu, Caie[1,2] Fan, Gongjian[1] Yang, Jing[4] Pan, Wenting[5]

第一作者：Li, Tingting

通信作者：Wu, CE[1]

机构：[1]Nanjing Forestry Univ, Coll Light Ind & Food Engineer, Dept Food Sci & Technol, Nanjing 210037, Jiangsu, Peoples R China;[2]Nanjing Forestry Univ, Coinnovat Ctr Substainable Forestry Southern Chin, Nanjing 210037, Jiangsu, Peoples R China;[3]Anhui Normal Univ, Coll Environm Sci & Engn, Wuhu 241000, Peoples R China;[4]Nanjing Forestry Univ, Adv Anal & Testing Ctr, Nanjing 210037, Jiangsu, Peoples R China;[5]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Xinyu 336600, Peoples R China

年份：2019

卷号：136

起止页码：284-295

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20240815604370);Scopus(收录号：2-s2.0-85067293075);WOS:【SCI-EXPANDED(收录号:WOS:000482533000028)】；

基金：This research was funded by the Natural Science Foundation of Jiangsu Province (Grants No. BK20150883), Youth Science and Technology Innovation Foundation of Nanjing Forestry University (CX2017020), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

语种：英文

外文关键词：Glycoprotein; Camellia oleifera Abel; Anticancer activity; Immunopotentiation; Hepatic carcinoma

摘要：Glycoproteins are naturally occurring biological macromolecule that possess known pharmacological properties. This study investigated the anticancer potential of a new glycoprotein, COG2a with the weight-average molecular weight of 25.9 kDa, isolated from Camellia oleifera Abel seeds by multiple column chromatography and the mechanism of anticancer action. MIT assay showed that the maximum inhibition rate of COG2a on HepG2 cells was 92.1%. Electron microscopic and fluorescence microscopy observation indicated that the HepG2 cells treated with COG2a exhibited typical apoptotic morphological character. Flow cytometry manifested that COG2a induced the cell cycle arrest at the G2 phases and decreased in mitochondrial membrane potential on the HepG2 cells. Western blotting exhibited that COG2a increased the expressions of the pro-apoptotic protein caspase-3 and Bax and decreased the expression of the anti-apoptotic proteins Bcl-2. Additionally, COG2a exerted an obvious anticancer effect on tumor-bearing mice and the maximum tumor inhibition rate is 72.77%. It also can promote the mouse thymus and pancreas index, the number of T lymphocytes cell subset and interferon-gamma to increase. These results indicate that COG2a exhibits in vitro and in vivo anticancer activities that associate with its immunopotentiation properties and may serve as a potential anticancer agent. (C) 2019 Elsevier B.V. All rights reserved.