文献类型：期刊文献

英文题名：Rejuvenation remadels transcriptional network to improve rhizogenesis in mature Juglans tree

作者：Song, Xiaobo[1] Liu, Hao[1] Bu, Dechao[2] Xu, Huzhi[3] Ma, Qingguo[1] Pei, Dong[1]

第一作者：宋晓波

通信作者：Pei, D[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,Natl Forestry &, 1958 Box, Beijing 100091, Peoples R China;[2]Chinese Acad Sci, Inst Comp Technol, 6 Kexueyuan South Rd Zhongguancun, Beijing 100190, Peoples R China;[3]Forestry Bur Luoning Cty, Luoyang City 471700, Henan, Peoples R China

年份：2021

卷号：41

期号：10

起止页码：1938-1952

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000710987200013)】；

基金：This work was supported by the National Key Research and Development Program (grant no. 2018YFD1000604), and the National Natural Science Foundation of China (grant no. 31672126).

语种：英文

外文关键词：adventitious root; BGL12; gene regulatory network; Juglans; rejuvenation

摘要：Adventitious rooting of walnut species (Juglans L.) is known to be rather difficult, especially for mature trees. The adventitious root formation (ARF) capacities of mature trees can be significantly improved by rejuvenation. However, the underlying gene regulatory networks (GRNs) of rejuvenation remain largely unknown. To characterize such regulatory networks, we carried out the transcriptomic study using RNA samples of the cambia and peripheral tissues on the bottom of rejuvenated and mature walnut (Juglans hindsii x J. regia) cuttings during the ARF. The RNA sequencing data suggested that zeatin biosynthesis, energy metabolism and substance metabolism were activated by rejuvenation, whereas photosynthesis, fatty acid biosynthesis and the synthesis pathways for secondary metabolites were inhibited. The inter- and intra-module GRNs were constructed using differentially expressed genes. We identified 35 hub genes involved in five modules associated with ARE Among these hub genes, particularly, beta-glucosidase-like (BGLs) family members involved in auxin metabolism were overexpressed at the early stage of the ARE Furthermore, BGL12 from the cuttings of Juglans was overexpressed in Populus alba x P. glandulosa. Accelerated ARF and increased number of ARs were observed in the transgenic poplars. These results provide a high-resolution atlas of gene activity during ARF and help to uncover the regulatory modules associated with the ARF promoted by rejuvenation.