文献类型：期刊文献

英文题名：Optimization of Factors Influencing Adventitious Rooting in Hybrid Larch

作者：Li, Kuipeng[1,2,3] Han, Hua[1] Xie, Yunhui[1] Sun, Xiaomei[1]

第一作者：Li, Kuipeng

通信作者：Sun, XM[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry &, Beijing 100091, Peoples R China;[2]Guangxi Forestry Res Inst, Guangxi Key Lab Super Timber Trees Resource Culti, Nanning 530002, Peoples R China;[3]Guangxi Forestry Res Inst, Forestry Minist China, Key Lab Cent South Fast Growing Timber Cultivat, Nanning 530002, Peoples R China

年份：2021

卷号：90

期号：2

起止页码：583-593

外文期刊名：PHYTON-INTERNATIONAL JOURNAL OF EXPERIMENTAL BOTANY

收录：;Scopus(收录号：2-s2.0-85102116882);WOS:【SCI-EXPANDED(收录号:WOS:000616252800019)】；

基金：This research was funded by The National Key Research and Development Program of China (Project No. 2017YFD0600401).

语种：英文

外文关键词：Hybrid larch; adventitious root; orthogonal test; variance analysis

摘要：Optimization of in vitro adventitious root induction contributes to the development of a large-scale production system of hybrid clone seedlings of larch (Larix spp.). We used orthogonal testing to investigate the factors that affect the rooting of hybrid larch shoots-activated carbon, vitamin B1, sucrose, glycine, glutamic acid (Glu), cysteine, pH, and induction time in the dark and indole-3-butyric acid (IBA). Variance analysis showed that the effects of sucrose concentration on rooting rate, survival rate, and average root number, pH on the average number of roots; and Glu on survival rate were all significant (p < 0.05) when compared to the mock treatments. Among all of the 45 treatments, the highest rooting rate was 90%, the highest survival rate was 100%, and the highest average root number was 11.8. The theoretically optimal procedure of in vitro root induction of hybrid larch shoots is shoot induction on L9 modified medium (pH 6.7) containing 2 mg.L-1 naphthalene acetic acid, 0.25 mg.L-1 IBA, 1/3 macroelements, 7.5 g.L-1 sucrose, and 5 mg.L-1 Glu for 8 days, and then root induction on L9 modified medium without auxin.