文献类型：期刊文献

中文题名：松针刺盘孢菌侵染对薄壳山核桃不同抗性品种酶活性和酚类物质的影响

英文题名：Effects of Colletotrichum fioriniae Infection on Enzyme Activity and Phenolics Content of Different Resistant Varieties of Pecan

作者：常君[1,2] 姚小华[1] 张亚波[1] 任华东[1] 张潇丹[1,3] 张成才[1] 万红卫[4]

第一作者：常君

机构：[1]中国林业科学研究院亚热带林业研究所,浙江富阳311400;[2]南京林业大学研究生院,南京210037;[3]西南大学资源环境学院,重庆400715;[4]浙江省建德市林业局,浙江建德311600

年份：2022

卷号：44

期号：3

起止页码：52-58

中文期刊名：西南大学学报：自然科学版

外文期刊名：Journal of Southwest University(Natural Science Edition)

收录：CSTPCD;;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：中国林业科学研究院中央级公益性科研院所基本科研业务费专项资金项目(CAFYBB2020SY014);国家重点研发专项(2019YFD1001603)。

语种：中文

中文关键词：薄壳山核桃;黑斑病;酶活性;抗性

外文关键词：pecan;black spot;enzymatic activity;resistance

分类号：S432.44;S664.9

摘要：以薄壳山核桃高抗品种“Kanza”和高感品种“Mahan”为研究对象,对比分析松针刺盘孢菌(Colletotrichum fioriniae)侵染后超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、多酚氧化酶(PPO)活性以及总酚和黄酮质量分数的变化.结果表明:不同酶活性和非酶类化合物质量分数在高抗与高感品种间表现出不同的变化动态.从侵染之初,高抗“Kanza”品种SOD酶活性就一直高于高感“Mahan”品种;POD酶和PPO酶活性随着侵染时间的延长,逐渐高于高感品种;CAT酶活性在侵染之初表现出下降的趋势,随着侵染时间的延长其活性逐渐升高,并在侵染后期升至最高;总酚和黄酮质量分数除侵染的第6 d外,其他时段高抗品种均明显高于高感品种.综上所述,薄壳山核桃抵御黑斑病病原菌侵染是多种酶和非酶类化合物共同参与的结果,SOD活性响应最为迅速,可能是最关键的防御松针刺盘孢菌侵染的活性氧清除物质.
The activities of SOD,POD,CAT,PPO and the mass fraction of total phenols and flavonoids of Colletotrichum fioriniae were compared and analyzed with the highly resistant variety‘Kanza’and highly susceptible variety‘Mahan’as the research subjects.The results showed that different enzyme activities and mass fractions of non-enzyme compounds presented different dynamics between high-resistant and high-susceptible varieties.In highly resistant variety‘Kanza’,SOD enzyme activity was higher than that of in highly susceptible variety‘Kanza’from the beginning of infection.POD and PPO enzyme activities were gradually higher than those of highly susceptible variety with the extension of infection time.CAT enzyme activity showed a decreasing trend at the early stage of the infection,but gradually increased with the extension of the infection time,and reached the highest in the later stage of the infection.The total phenols and flavonoids contents in other periods of time except the 6 th day of infection in highly resistant variety were significantly higher than those of in highly susceptible variety.The resistance of pecan plant to black spot pathogen infection involves the participation of multiple enzymes and non-enzymatic compounds.The SOD activity is the most responsive,with extremely significant differences in its activities.It may be the most critical active oxygen scavenging system for defense against C.fioriniae infection.