文献类型：期刊文献

英文题名：Identification of Key Lipogenesis Stages and Proteins Involved in Walnut Kernel Development

作者：Jin, Feng[1] Zhou, Ye[1] Zhang, Pu[1] Huang, Ruimin[1] Fan, Wei[2] Li, Baoxin[1] Li, Guangzhu[1] Song, Xiaobo[1] Pei, Dong[1]

第一作者：Jin, Feng

通信作者：Pei, D[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China

年份：0

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20231013659704);Scopus(收录号：2-s2.0-85149136741);WOS:【SCI-EXPANDED(收录号:WOS:000942687000001)】；

基金：Funding This research is financially supported by the State Key Laboratory of Tree Genetics and Breeding support program (Grant No. CAFYBB2019ZY001) .

语种：英文

外文关键词：walnut; lipogenesis; proteomics; oleosin; transgenic

摘要：Walnuts are abundant in oil content, especially for polyunsaturated fatty acids, but the understanding of their formation is limited. We collected walnut (Juglans regia L.) kernels at 60, 74, 88, 102, 116, 130, and 144 days after pollination (designated S1-S7). The ultrastructure and accumulation of oil bodies (OBs) were observed using transmission electron microscopy (TEM), and the oil content, fatty acid composition, and proteomic changes in walnut kernels were determined. The oil content and OB accumulation increased during the development and rose sharply from S1 to S3 stages, which are considered the key lipogenesis stage. A total of 5442 proteins were identified and determined as differentially expressed proteins (DEPs) using label-free proteomic analysis. Fatty acid desaturases (FAD) 2, FAD3, oleosin, and caleosin were essential and upregulated from the S1 to S3 stages. Furthermore, the highly expressed oleosin gene JrOLE14.7 from walnuts was cloned and overexpressed in transgenic Brassica napus. The overexpression of JrOLE14.7 increased the oil content, diameter, hundred weight of seeds and changed the fatty acid composition and OB size of Brassica napus seeds. These findings provide insights into the molecular mechanism of oil biosynthesis and the basis for the genetic improvement of walnuts.